下丘脑室旁核微量注射P物质对豚鼠呼吸的影响-东南大学学报医学版

下丘脑室旁核微量注射P物质对豚鼠呼吸的影响

单位：东南大学基础医学院,生理学与药理学系,江苏,南京,210009

分类号：R332, R-33

出版年·卷·期（页码）：2004·23·第二期（89-92）

摘要：

目的:探讨下丘脑室旁核(paraventricular nuclei,PVN)微量注射P物质(substance P,SP)对豚鼠呼吸运动的影响及其作用机制.方法:采用中枢立体定位技术,在豚鼠PVN预先植入带芯的不锈钢外套管.实验时切断双侧迷走神经,自主呼吸,通过内管恒速注射不同药物,持续记录呼吸流量、动脉血压、膈肌肌电活动.结果:与生理盐水组比较,微量注射SP后2～27min,膈肌肌电活动增强,通气量增加(均为P＜0.001).微量注射SP后3～20min,血压升高(P＜0.05).若先以SP的受体拮抗剂[D-Pro2,D-Trp7,9]-SP(DPDTDT)或血管升压素(vasopressin,VP)的V1受体拮抗剂[deamino-Pen1,Val4,D-arg8]-VP(DPVAVP)预处理,则SP的呼吸和血压效应消失.单独注射DPDTDT或DPVAVP对呼吸和血压没有显著影响.结论:PVN内微量注射SP有呼吸兴奋作用,此作用可能是由NK1受体或V1受体介导的.

Objective TO investigate the effects and mechanisms of microinjection of substance P(SP) into the hypothalamic paraventricular nuclei (PVN) on respiration in guinea pigs.Methods Experiments were carried out on 36 anesthetized guinea pigs of both sexes,vagotomized and spontaneously breathing,with stainless steels implanted into PVN 24 h before.The drugs were injected into left PVN via the steels,then the effects of respiration and arterial blood pressure were observed for 40?min.Results Compared with NS,SP enhanced both respiration during 2～27?min ( P <0.001) and blood pressure during 3～20?min ( P <0.05).Pretreatment with NK 1 antagonist (DPDTDT) of SP or V 1 antagonist (DPVAVP) of vasopression,SP had no such effects.Neither DPDTDT nor DPVAVP singly had such effects.Conclusion Microinjection of SP into PVN induced respiratory excitement which was likely obtained via NK 1 and (or) V 1 receptors.

参考文献：

[1] 刘婧, 姜恩魁. P物质和P物质受体. 锦州医学院学报2001(1). doi:10.3969/j.issn.1674-0424.2001.01.036
[2] 虞燕琴, 夏强. 下丘脑室旁核的心血管调节功能及与心血管疾病的关系. 浙江大学学报(医学版)2001(3). doi:10.3785/j.issn.1008-9292.2001.03.017
[3] TINDAL J S. The forebrain of the guinea pig in stereotaxic coordinates. 1965. doi:10.1002/cne.901240210
[4] HednerT. Evidence suggesting a role for substance P in central respiratory regulation in the rat. 1981. doi:10.1111/j.1748-1716.1981.tb06848.x
[5] MAZZONE S B, HINRICHSER C F, GERAGHTY D P. Substance P receptors in brain stem respirtory centers of the rat: regulation of NK1 receptors by hypoxia, 1997(3)
[6] 朱长庚, 张喜京, 刘庆莹. 下丘脑室旁核神经元多重神经支配的电镜研究, 1994(2)
[7] KC P, HAXHIU M A, TOLENTINO-SILVA F P. Paraventricular vasopressin-containing neurons project to brain stem and spinal cord respiratory-related sites. 2002(1-2). doi:10.1016/S1569-9048(2)00131-3
[8] 段晓勤, 鞠躬, 饶志仁. P物质对大鼠下丘脑催产素及加压素免疫反应性神经元Fos癌蛋白的诱导作用. 解剖学报1998(3)
[9] 方秀斌, 佟晓杰, 李松林. 哮喘豚鼠内脏感觉传人部位P物质含量的变化. 中国病理生理杂志1999(10)
[10] 楚东龄, 宋俊峰, 饶志仁. 哮喘豚鼠感觉神经节内SP表达变化. 第四军医大学学报2002(13)

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