Objective  To investigate the function of pine pollen on the proliferation activity of human lung fibroblast,and try to explore the mechanism of pine pollen.Methods  According to densities of pine pollen,cells were divided into different groups,and cells without pine pollen treated were selected as control group.After human lung fibroblast was treated with pine pollen,the growth rate of cell was observed at different time.Trypan blue dyeing was used to measure death rate of cell.Results  Pine pollen significantly  improved growth of cells and decreased the amount of dead cell.Conclusion  Pine pollen influences on cell proliferation by improving cell growth.