大鼠孤束核内H3受体对哮喘发作时脑组织中Fos蛋白表达的影响-东南大学学报医学版

大鼠孤束核内H3受体对哮喘发作时脑组织中Fos蛋白表达的影响

单位：1.南京胸科医院,六病区,江苏,南京,210029； 2.东南大学基础医学院,生理学与药理学系,江苏,南京,210009

分类号：R562.25

出版年·卷·期（页码）：2005·24·第六期（371-374）

摘要：

目的:探讨大鼠孤束核(NTS)内组胺H3受体对哮喘发作时大脑Fos蛋白表达的影响及其意义.方法:取健康雄性SD大鼠制备哮喘模型并诱发哮喘发作,采用中枢立体定位及微量注射技术,分3组分别在NTS内微量注射无菌生理盐水、H3受体激动剂R-α-methylhistamine(RMHA)以及H3受体拮抗剂thioperamide(Thio)加RMHA,用SABC免疫组织化学方法测定大鼠哮喘急性发作后1 hFos蛋白在丘脑至延髓平面各核团的分布情况.结果:与哮喘组相比,哮喘大鼠NTS内微量注射1μgRMHA,丘脑室旁核、下丘脑室旁核、室周核、NTS等核团Fos蛋白表达减少,阳性细胞数降低,差异有显著性(P＜0.01);而用Thio 5μg预处理则可拮抗RMHA的上述效应.结论:NTS内H3受体可能通过丘脑室旁核、下丘脑室旁核和室周核等脑区相关神经元参与对哮喘的神经调控,从而可能抑制哮喘发作.

Objective To explore the effect of histamine H_3 receptors in nucleus tractus solitarius(NTS) on c-Fos expression in the brain of rats with asthma onset.Methods Male Sprague-Dawley(SD) rats were sensitized with ovalbumin to induce asthma using central stereotaxic and microinjection techniques.The model rats were randomly divided into three groups,injected NS,R-α-methylhistamine(RMHA,a H_3 receptor receptors agonist),and thioperamide(Thio,a H_3 receptors antagonist)+RMHA into NTS respectively.Fos positive cells distributed in different nucleus were detected by immunohistochemistry SABC method after the challenge one hour later.Results After microinjection 1?μg RMHA into NTS at the onset of asthma,the number of Fos positive cell and Fos density presented in NTS,periventricular hypothalamic nucleus(Pe),hypothalamic paraventricular nucleus(Pa),and raraventricular thalamic nucleus(PVN) decreased markedly((P<)(0.01)).These effects can be blocked by pre-treatment with 5?μg microinjection of Thiop into NTS.Conclusion This study suggests that histamine H_3 receptors in NTS can ameliorate asthma via regulating some neurons in Pa,Pe and PVN.

参考文献：

[1] SAGAR S M, SHARP F R. Early response genes as markers of neuronal activity and growth factor action, 1993
[2] KLECHA A J, GENARO A M, LYSIONEK A E. Experimental evidence pointing to the bidirectional interaction between the immune system and the thyroid axis. 2000(7)
[3] 杨志军, 葛煦, 饶志仁. Fos蛋白在LPS免疫激发大鼠脑内的分布. 解剖学报2000(4)
[4] 董榕, 张敏. 哮喘豚鼠脑内组胺含量变化及中枢组胺H3受体的调节作用. 现代医学2003(3). doi:10.3969/j.issn.1671-7562.2003.03.005
[5] BROWN R E, STEVENES D R, HAAS H L. The physiology of brain histamine. 2001(6)
[6] 杨志军, 饶志仁, 鞠躬. 膈下迷走神经切断术抑制腹腔给予LPS诱导的延髓内脏带内Fos表达. 解剖学报2000(2)

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