大鼠急性坏死性胰腺炎时P物质在肠壁上的表达及其与肠黏膜通透性的关系-东南大学学报医学版

大鼠急性坏死性胰腺炎时P物质在肠壁上的表达及其与肠黏膜通透性的关系

单位：1.北京市大兴区医院,普外科,北京,102600； 2.东南大学附属中大医院,普外科,江苏,南京,210009

分类号：R657.51

出版年·卷·期（页码）：2005·24·第六期（359-363）

摘要：

目的:了解急性坏死性胰腺炎(ANP)时大鼠回肠组织中P物质的表达及其与肠黏膜通透性的关系.方法:健康成年SD大鼠随机分为对照组40只和ANP组40只,对照组开腹后只翻动胰腺,ANP组大鼠胰胆管恒速逆行注射5%牛黄胆酸钠制成ANP大鼠模型,分别于制模成功后8、12、16和20h处死大鼠采取标本.用同位素法测定肠黏膜通透性,免疫组织化学方法检测P物质在回肠组织中的表达水平和组织学定位.结果:ANP组大鼠回肠组织中P物质的表达以及肠黏膜通透性(99mTc-DTPA排泄率)较对照组明显增加(P＜0.05).P物质在回肠组织中的表达与肠黏膜通透性呈明显正相关(r=0.66,P＜0.01).结论:ANP时大鼠回肠组织中P物质表达升高,且随时间延长而增加,其表达与大鼠肠黏膜通透性呈正相关.

Objective To investigate the expression of substance P in rats’ intestinal tissue and study the relation between substance P and mucosa permeability in acute necrotizing pancreatitis(ANP).Methods 80 adult Sprague-Dawley rats were randomly divided into control group((n=)40) and ANP group((n=)40).Sacrifices were made in 8,12,16 and 20?h later in every group after ANP model was established by injecting 5% sodium taurocholate into pancreatic duct respectively.The intestinal mucosal permeability and the expression of substance P were studied.Results In ANP groups,intestinal mucosal permeability and the expression of substance P were significantly higher than those in the control group((P<)0.05).The overexpression of substance P was correlated with the intestinal mucosal permeability in ANP(r=0.66,P<(0.01)).Conclusion The expression of substance P and the permeability in intestine of rats are increased significantly in ANP.There exists a possitive correlation between substance P and the intestinal permeability.

参考文献：

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