大鼠多发性脑梗死后脑组织ICAM-1蛋白和NOS阳性神经元表达的变化-东南大学学报医学版

大鼠多发性脑梗死后脑组织ICAM-1蛋白和NOS阳性神经元表达的变化

单位：1.常州市第二人民医院,神经内科,江苏,常州,213003； 2.东南大学医学院,神经生物学研究所,江苏,南京,210009； 3.南京中医药大学,中医基础理论教研室,江苏,南京,210049

分类号：R743, R-332

出版年·卷·期（页码）：2005·24·第五期（321-324）

摘要：

目的:观察大鼠多发性脑梗死后脑组织内皮细胞表达细胞间粘附分子-1(ICAM-1)蛋白和一氧化氮合酶(NOS)阳性神经元表达的时程变化.方法:48只健康雄性SD大鼠制成多发性脑梗死模型,梗死后分别于1 h到14 d不同时间点处死并取脑,应用免疫组织化学法和NADPH-d组化法标记脑组织切片ICAM-1蛋白和NOS阳性神经元,设对照组和正常组,各6只大鼠.结果:多发性脑梗死后2 h ICAM-1蛋白表达达到高峰,NOS阳性神经元表达也同样达到高峰.结论:ICAM-1和NOS神经元参与大鼠多发性脑梗死的脑损伤.

Objective To investigate the changes of NOS positive neuron and ICAM?1 protein expression of ischemia brain induced by embolization in rats.Methods Male SD rats(n=48) were subjected to multi-focal cerebral infarct,then brain tissues were obtained at 1h to 14?d accordingly after the onset of embolization,6 additional normal and control animals were employed as controls respectively.The NOS and ICAM?1 protein expression in brain sections were studied with immunohistochemical and histochemical methods.Result ICAM?1,as well as NOS expression,reached the maximum at 2 h,NOS expression was equivalent in this study,but NOS expression did not change much.Conclusion NOS and ICAM?1 commonly participate in cerebral ischemia injury.

参考文献：

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