兔下颌角截骨后咬肌肌球蛋白重链mRNA表达的变化-东南大学学报医学版

兔下颌角截骨后咬肌肌球蛋白重链mRNA表达的变化

单位：东南大学附属中大医院,整形外科,江苏,南京,210009

分类号：R622, R-332

出版年·卷·期（页码）：2005·24·第五期（304-306）

摘要：

目的:通过对兔下颌角弧形截骨后咬肌肌球蛋白重链(MHC)mRNA测定,研究咬肌在骨架缩短后的适应性变化规律.方法:3月龄雌性新西兰大白兔25只,行左侧下颌角弧形截骨,右侧不手术作自身对照.2、4、8、12和24周分别处死5只动物,提取咬肌MHCⅠ、MHCⅡ型和内参GAPDH的mRNA进行RT-PCR.结果:手术侧较对侧MHCⅠ型的mRNA转录降低,12周后正常.MHCⅡmRNA-2周时先降低,4周后恢复正常.2、4、8周MHCⅡ/MHCⅠ手术侧较对侧增加.结论:下颌骨架缩短后咬肌发生适应性的改建,咬肌组分的这种变化有利于建立口颌系统新的平衡和咀嚼功能的恢复.

Objective To study the changing feature of massester muscle by measuring expression of myosin heavy chain mRNA after ostectomy of the mandibular angle along arc line.Methods Ostectomy of the left mandibular angle were performed on 25 New Zealand female rabbits aged three months.The unoperated right lateral was taken as the control.5 rabbits were sacrificed at the following intervals: 2nd,4th,8th,12th and 24th week.Massester immediately was dissected out and stored at-70?℃,subsequently the mRNAs of MHCⅠ,MHCⅡ and GAPDH were measured by RT?PCR.Results The amount of MHCⅠ mRNA on the left massester significantly decreased at the first 8 week.The amount of MHCⅡ mRNA was significantly decreased at the 2nd week,and then recovered after 4 weeks.MHCⅡ/MHCⅠ on the left masseter was significantly increased at the 2nd,4th,8th week.Conclusion The reconstruction of massester muscle after ostectomy of the mandibular angle is beneficial for reforming new balance of gnathostomatic system,thus restoring chewing function.

参考文献：

[1] SONG H S, PARK C G. Masseter muscle atrophy after ostectomy of the mandibular angle in rabbits, 1997(1)
[2] ALBIS A, JANMOT C, BECHET J J. Comparison of myosins from the masseter muscle of adult rat,mouse and guinea-pig persistence of neonatal-type isoforms in murine muscle, 1986(2)
[3] GOLDSPINK G, SCUTT A, LOUGHNA P T. Gene expression in skeletal muscle in response to stretch and force gene ration, 1992
[4] READER M, SCHWARTZ G, ENGLISH A W. Brief exposure to testosterone is sufficient to induce sex differences in rabbit masseter muscle. 2001. doi:10.1159/000047884
[5] LAMBERT C D. Hypertrophy of branchial muscles, 1976

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