咖啡对糖尿病大鼠膀胱功能障碍的影响-东南大学学报医学版

咖啡对糖尿病大鼠膀胱功能障碍的影响

单位：1.南京大学医学院附属鼓楼医院,泌尿外科,江苏,南京,210008； 2.东南大学基础医学院,生物化学教研室,江苏,南京,210009； 3.南京大学医学院附属鼓楼医院,内分泌科,江苏,南京,210008

分类号：R587.2, R-332

出版年·卷·期（页码）：2005·24·第五期（300-303）

摘要：

目的:探讨咖啡对糖尿病大鼠膀胱功能障碍的影响及作用机制.方法:链脲佐菌素腹腔注射诱导糖尿病模型.SD大鼠随机分为糖尿病组(DM组)、糖尿病咖啡治疗组(Coffee组)及正常对照组(NC组).7周后测定各组膀胱湿重量、阈容量、相对排尿量(V%)、膀胱环磷酸腺苷(cAMP)含量及逼尿肌收缩功能的变化.结果:Coffee组与DM组相比,膀胱湿重量与阈容量显著降低,V%与膀胱cAMP含量明显升高.DM组逼尿肌肌条的收缩功能明显受损,咖啡治疗后肌条收缩性有所改善.结论:咖啡可能通过其中的咖啡因升高膀胱cAMP浓度、改善膀胱充盈感觉和逼尿肌收缩性等机制来防治糖尿病大鼠膀胱病变.

Objective To explore the effects and mechanisms of coffee on bladder dysfunction in the diabetic rats.Methods SD rats were divided at random into DM group,Coffee group and NC group.Diabetic rat model was induced by injection of streptozotocin into peritoneal cavity.After 7 weeks,the bladder wet weight,volume threshold for micturation,V%,cAMP concentration of bladder and the changes of the detrusor strip contractility were measured and compared among the groups.Results Compared with DM group,the bladder wet weight and volume threshold for micturation were significantly reduced in Coffee group while V% and cAMP concentration of bladder were significantly increased.Moreover,the diabetic rats treated with coffee can significantly ameliorate the impaired bladder detrusor contractility.Conclusion These results indicate that coffee may have a curative effect on bladder function of diabetic rats by increasing cAMP content, improving the sensation of bladder fullness and the detrusor contractility.

参考文献：

[1] MUMTAZ F H, THAMPSON C S, KHAN M A. Alterations in the formation of cyclic nucleotides and prostaglandins in the lower urinary tract of the diabetic rabbit. 1999(6). doi:10.1007/s002400050137
[2] SALAZAR-MARTINEZ E, WILLETT W C, ASCHERIO A. Coffee consumption and risk for type 2 diabetes mellitus. 2004
[3] 王尧, 石凤英. 灵芝对糖尿病大鼠代谢紊乱及早期蛋白尿的作用. 东南大学学报(医学版)2003(3). doi:10.3969/j.issn.1671-6264.2003.03.008
[4] SMITH P F, SMITH A, MINERS J. Report from the expert working group on the safety aspects of dietary caffeine, 2000
[5] FREDHOLM B B, BATTIG K, HOLMAN J. Actions of caffeine in the brain with special reference to factors that contribute to its widespread use, 1999
[6] 龚宇, 熊恩庆, 金锡御. 糖尿病大鼠膀胱CGRP神经病变的研究. 中华泌尿外科杂志2000(10)
[7] 苏兴文, 皮荣标, 林穗珍. 咖啡因对谷氨酸诱导神经元凋亡的保护作用. 中国药理学通报1999(6)
[8] TONG Y C, CHENG J T. Alteration of M(3) subtype muscarinic receptors in the diabetic rat urinary bladder. 2002(3). doi:10.1159/000056164

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