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维生素C增强As2O3诱导宫颈癌HeLa细胞凋亡的研究
作者：曹娟 郑杰 吕秀宁 
单位：东南大学基础医学院,病理学与病理生理学系,江苏,南京,210009
关键词：维生素C 三氧化二砷 HeLa细胞 细胞凋亡 端粒酶 
分类号：R737.33
出版年·卷·期（页码）：2005·24·第五期（294-299）
摘要：

目的:探讨维生素C是否能影响三氧化二砷(As2O3)诱导宫颈癌HeLa细胞凋亡,以及它对细胞内端粒酶活性的影响.方法:用噻唑蓝(MTT)法筛选出维生素C的最佳浓度250μmol·L-1,并用该浓度联合不同浓度的As2O3同时处理HeLa细胞后,用光镜、MTT法和流式细胞术研究细胞生长和凋亡的情况,用TRAP-ELISA法检测细胞内端粒酶活性的变化.结果:单独1μmol*L-1As2O3处理过的细胞生长未受到明显抑制,但联合250μmol·L-1维生素C处理细胞,则细胞生长受到抑制,且部分细胞出现凋亡形态学特征.2、5、10μmol·L-1 As2O3联合维生素C用药组的细胞凋亡率也比单独用As2O3组明显增高,且出现凋亡的时间提早.端粒酶活性检测结果提示,联合用药组细胞内端粒酶活性均明显比单独用As2O3组低.结论:小剂量维生素C能增强As2O3诱导宫颈癌HeLa细胞凋亡,并能增强As2O3对细胞内端粒酶活性的抑制作用.

Objective To study whether ascorbic acid can enhance arsenic trioxide to induce apoptosis and to inhibit telomerase activity in cervical cancer HeLa cells.Methods After HeLa cells were treated with various concentrations of As_2O_3 plus 250 μmol·L^{-1} ascorbic acid,effects of As_2O_3and ascorbic acid on survival and apoptosis of HeLa cells were determined by light microscope,MTT assay,flow-cytometric analysis.Telomerase activity in HeLa cells was determined by TRAP?ELISA.Results There was no marked effect on the survival of HeLa cells after the cells treated by 1 μmol·L^{-1} As_2O_{3.}However,after 1 μmol·L^{-1} As_2O_3plus 250 μmol·L^1 ascorbic acid treated HeLa cells,significant growth inhibition and apoptosis characteristics were found.As to the higher concentrations of As_2O_3(2,5,10 μmol·L^{-1}),the apoptosis rate of HeLa cells treated by(As_2O_3)plus ascorbic acid was higher than that of HeLa cells treated by As_2O_3alone and apoptosis characteristics were earlier.Meanwhile,there was more marked telomerase inhibition following to As_2O_3plus 250?μmol·L^{-1} ascorbic acid treatment than As_2O_3alone.Conclusion Ascorbic acid enhances arsenic trioxide to induce apoptosis in HeLa cells,which is related to its inhibiting telomerase activity in HeLa cells.
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