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BXSB小鼠肾组织中MCP-1的异常表达及甲泼尼龙的干预作用
作者：孙茹蓉 王美美 朱小霞 
单位：东南大学附属中大医院,风湿科,江苏,南京,210009
关键词：狼疮肾炎 单核细胞化学吸引蛋白1 甲泼尼龙 小鼠 
分类号：R-332, R593.242, R977.11
出版年·卷·期（页码）：2005·24·第四期（229-232）
摘要：

目的:探讨BXSB狼疮鼠肾组织单核细胞趋化因子-1(MCP-1)的表达情况及甲泼尼龙(MPS)是否可通过抑制MCP-1的表达而缓解狼疮肾炎(LN).方法:将18周龄雄性BXSB自发性狼疮小鼠随机分成BXSB治疗组(n=6)和对照组(n=6);另随机抽取同龄雄性BALB/c小鼠6只为正常对照组.MPS组小鼠每日腹腔注射MPS25mg·kg-1;BXSB小鼠对照组及BALB/c小鼠按体质量每日同一时间腹腔注射等体积生理盐水,连续3周.用逆转录-聚合酶链反应(RT-PCR)研究BXSB小鼠肾脏MCP-1mRNA的表达,并观察小鼠24 h尿蛋白排泄量.结果:BXSB治疗组与对照组小鼠肾组织均检测到MCP-1 mRNA的表达,而BALB/c正常对照组小鼠未见表达;BXSB治疗组小鼠肾组织MCP-1mRNA表达量显著低于对照组(P＜0.01);BXSB治疗组24 h尿蛋白排泄量显著低于对照组(P＜0.01).结论:MCP-1可能参与介导LN的发生、发展;MPS可能通过抑制肾组织MCP-1的表达而减轻蛋白尿,从而缓解LN.

Objective To investigate whether monocyte chemoattractant protein-1(MCP 1) mediates lupus nephritis(LN) in BXSB mice and whether methylprednisolone (MPS) ameliorates LN by inhibiting MCP 1 expression. Methods 18-week-old male BXSB mice (n=6) displaying clinical symptoms of glomerulonephritis were treated(i.p.) for 3 weeks with MPS (25 mg·kg -1·d -1) dissolved in NS. BXSB controls were age- and sex-matched BXSB mice (n=6) receiving NS alone. Age- and sex- matched BALB/c mice (n=6) were used as normal controls that were treated in the same way as the BXSB controls. MCP 1 expression was investigated by means of RT PCR. Proteinuria was also evaluated. Results MCP 1 was strongly expressed in kidneys of all BXSB mice, while no signals of expression were found in kidneys of BALB/c mice. MCP 1 expression in kidneys of mice in MPS group dramatically reduced in comparison with that of BXSB control mice( P<0.01). Compared with BXSB control mice, 24 h total amount of urinary protein of mice in MPS groups significantly decreased after three weeks’ treatment( P<0.01). Conclusion MCP 1 may mediate LN in BXSB mice. MPS can reduce urinary protein and ameliorate LN of BXSB mice, partly via inhibiting MCP 1 expression in kidney.
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