甘草对大鼠胃动力功能影响的实验研究-东南大学学报医学版

甘草对大鼠胃动力功能影响的实验研究

单位：1.东南大学基础医学院,生理学与药理学系,江苏,南京,210009； 2.东南大学附属中大医院,中医内科,江苏,南京,210009

分类号：R282.7, R333.3

出版年·卷·期（页码）：2005·24·第四期（226-229）

摘要：

目的:探讨甘草对大鼠胃动力的作用及其机制.方法:观察甘草组与空白组移行性综合肌电(MMC)的周期持续时间、Ⅲ相持续时间、Ⅲ相每分钟快波数(FM)和每簇的快波数(FC)的变化;采用免疫组织化学法结合显微图像定量分析扫描系统检测胃嗜铬细胞及其肌间神经丛中5-羟色胺(5-HT)、P-物质(SP)、血管活性肠肽(VIP)的相对含量.结果:甘草组与空白组比较,MMCⅢ相FM和FC明显减少,MMC周期明显延长,Ⅲ相持续时间明显缩短,均有显著性差异(均P＜0.05).甘草组胃黏膜及肌间神经丛内5-HT表达明显较空白组减少(P＜0.05及P＜0.01),胃窦黏膜无明显SP、VIP阳性免疫反应物表达,但胃肌间神经丛内SP含量明显减少(P＜0.01)、VIP含量明显增加(P＜0.05).结论:甘草对大鼠胃动力有抑制作用,这种抑制作用可能与其引起5-HT、SP、VIP分泌失调有关.

Objective To investigate the effects of glycyrrhiza decoction on migrating myoelectric complex(MMC)and gastrointestinal hormone in stomach in rats.Methods MMC cycle,phase Ⅲ duration,fast wave numbers of phase Ⅲ of MMC in one minute,fast wave numbers of one cluster in phase Ⅲ of MMC were observed in glycyrrhiza group and control group rats with electrophysiology method,and immunohistochemistry was performed to examine relative content of serotonin(5 HT),substance P(SP) and vasoactive intestinal polypeptide(VIP) in gastric autrum mucous membrane chromophile(EC) and myenteric nerve plexus in stomaches of both control group and glycyrrhiza group rats. Results Compared with control group,glycyrrhiza group decreased fast wave numbers in one minute and fast wave numbers in one cluster in phase Ⅲ of MMC of stomach( P<0.05),and evidently prolonged gastric cycle of MMC(P<0.05),shortened the phase Ⅲ duration (P<0.05) or makes the phase Ⅲ of MMC absent. Comparisons between glycyrrhiza group and control group indicated that content of 5 HT in gastric mucous membrane and mytenteric nerve plexus was evidently decreased(P<0.05),and content of SP in mytenteric nerve plexus of stomach of rats was evidently decreased (P<0.05),and content of VIP in stomach of rats was evidently increased(P<0.05). Conclusion Glycyrrhiza is able to inhibit gastric motility,which relates with the amount of 5 HT,SP,VIP secreted by gastric mucous membrane of rats.

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