GDNF mRNA、GFR-α1 mRNA在不同年龄大鼠SVZ和海马表达的变化及其意义-东南大学学报医学版

GDNF mRNA、GFR-α1 mRNA在不同年龄大鼠SVZ和海马表达的变化及其意义

单位：1.东南大学基础医学院,人体解剖与组织胚胎学系,江苏,南京,210009； 2.江苏省人民医院,病理科,江苏,南京,210029； 3.中山大学第二附属医院,林百欣实验中心,广东,广州,510120

关键词：脑室管膜下区海马胶质细胞源性神经营养因子胶质细胞源性神经营养因子受体-α1 大鼠

分类号：R-33, Q954

出版年·卷·期（页码）：2005·24·第三期（171-175）

摘要：

目的:观察不同年龄SD大鼠脑室管膜下区(SVZ)和海马GDNF mRNA及其受体GFR-α1 mRNA表达水平的变化,探讨其在神经发生及脑老化中的作用.方法:用RT-PCR方法检测胚胎18 d、新生以及1、3、8、12月龄SD大鼠SVZ和海马GDNF mRNA及其受体GFR-α1 mRNA的变化.结果:随着年龄增长,SD大鼠SVZ和海马GDNF mRNA及其受体GFR-α1 mRNA表达水平逐渐降低.且各年龄组海马表达参数的幅度小于SVZ.结论:GDNF及其受体GFR-α1在神经发育及脑老化进程中起到一定的作用.

Objective To investigate the effect of GDNF and GFRα_1 in the subventricular zone and hippocampus on neural precursor proliferation,via analysing the gene expression of GDNFmRNA and GFRα_1mRNA from Sprague-Dawley rats of different ages. Methods The level of GDNFmRNA and GFR-α_1mRNA in the subventricular zone and hippocampus was observed and compared in rats of different age, using semi-quantitative RT-PCR method with β-actin as an inner consultation. Results With the age increased,the gene expression of GDNFmRNA and GFRα_1mRNA in the subventricular zone and hippocampus was obviously decreased, and the gene expression of GDNFmRNA and GFRα_1mRNA in the subventricular outnumbered those of gene expression in hippocampus of different ages. Conclusions The gene expression of GDNFmRNA and GFRα_1mRNA in the subventricular zone and hippocampus obviously decreases with the increase of age. GDNF and GFRα_1 exert important effects on the neural precursor proliferation, the decline of the neuronal compensatory ability and the neural plasticity of the aged brain.

参考文献：

[1] Doetsch F, CAILL I, LIM D A. Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. 1999. doi:10.1016/S0092-8674(0)80783-7
[2] Palmer T D, TAKAHASHI J, GAGE F H. The adult rat hippocampus contains primordial neural stem cells. 1997. doi:10.1006/mcne.1996.0595
[3] Gritti A, FROLICHSTHAL-SCHOELLER P, GALLI R. Epidermal and fibroblast growth factors behave as mitogenic regulator for a single multipotent stem cell-like population from the subventricular region of the adult mouse forebrain, 1999
[4] Arsenijevic Y, WEISS S. Insulin-like growth factor-1 is a differentiation factor for postmitotic CNS stem cell-derived neuronal precursors:distinct action from those of brain-derived neurotrophic factor, 1998
[5] LIN L F, DOHERTY D H, LILE J D. GDNF:a glia cell line-derived neurotrophic factor for midbrain dopaminergic neurons. 1993(5111). doi:10.1126/science.8493557
[6] Homma S, OPPENHEIM R W, YAGINUMA H. Expression pattern of GDNF,c-ret,and GFRalphas suggests novel roles for GDNF ligands during early organogenesis in the chick embryo, 2000
[7] Trupp M, SCOTT R, WHITTEMORE S R. Ret-dependent and ret-independent mechanisms of glial cell line-derived neurotrophic factor singaling in neuronal cells. 1999. doi:10.1074/jbc.274.30.20885
[8] SWANSON L W. Brain maps: structure of the rat brain, 1992
[9] 高殿帅, 蔡青, 鲁强. 成年大鼠脑内具有神经发生功能的脑室下区的细胞形态学研究. 神经解剖学杂志2004(2). doi:10.3969/j.issn.1000-7547.2004.02.002
[10] Jing S, WEN D, YU Y. GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-α,a novel receptor for GDNF. 1996. doi:10.1016/S0092-8674(0)81311-2
[11] 汪建民, 袁群芳, 姚志彬. 大鼠室管膜下区和齿状回神经前体细胞增殖的衰老性变化. 神经解剖学杂志2002(1). doi:10.3969/j.issn.1000-7547.2002.01.004
[12] KUHU H G, DICKINSON-ANSON H, GAGE F H. Neurogenesis in the dentate gyrus of the adult rat:age-related decrease of neuronal progenitor proliferation, 1996
[13] Golden P J, DEMARO A J, SBORNE A P. Expression of neurturin,GDNF,and GDNF family-receptor mRNA in the developing and mature mouse. 1999(2). doi:10.1006/exnr.1999.7127

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录