高效液相色谱电化学法测定血清褪黑激素水平-东南大学学报医学版

高效液相色谱电化学法测定血清褪黑激素水平

单位：1.东南大学临床医学院,中心实验室,江苏,南京,210009； 2.东南大学附属中大医院,内分泌科,江苏,南京,210009

分类号：O652.63

出版年·卷·期（页码）：2005·24·第二期（105-108）

摘要：

目的:建立测定血清褪黑激素水平的高效液相色谱方法.方法:血清褪黑激素用氯仿反复萃取,氯仿层经真空干燥、氮气吹干,最后残流物溶解进样.用C18反相柱进行色谱分析,电化学检测器测定结果.结果:测定线性范围达1.72nmol·L-1,最低检测限为0.084nmol·L-1,日内变异4.2%～4.8%,日间变异4.4%～5.0%,方法回收率55.3%～76.5%.结论:本方法可用于临床实验室测定血清褪黑激素水平.

Objective A high performance liquid chromatography (HPLC) method was established for determination of melatonin in human serum with electrochemical detection. Methods The serum melatonin was extracted repeatedly by the chloroform,and was dried under vacuum in the organic phase. The residue was dissolved by the chloroform and dried under a nitrogen stream at room temperature. At last the residue was dissolved by the eluent for HPLC. Results The linearity of this method was up to 1.72-nmol·L -1; the detection limit was 0.084-nmol·L -1; the within-day imprecision was 4.1%～4.8%;the between-day imprecision was 4.4%～5.0%;the recovery was 76.5%～82.4%. Conclusion This method can be used for the determination of serum melatonin in clinical laboratories.

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