人外周血来源的树突状细胞的体外诱导培养-东南大学学报医学版

人外周血来源的树突状细胞的体外诱导培养

作者：李曼¹ 陈宝安¹ 孙载阳¹ 李翠萍² 高冲¹ 王骏¹ 孙耘玉¹ 傅强² 陈津²

单位：1.东南大学附属中大医院,血液科,江苏,南京,210009； 2.南京红十字血液中心,江苏,南京,210092

分类号：Q343.6

出版年·卷·期（页码）：2005·24·第二期（90-93）

摘要：

目的:从健康人外周血中诱导高纯度树突状细胞(DC).方法:将外周血单个核细胞用贴壁法分离出单核细胞,经多细胞因子联合诱导出DC.结果:培养8～9 d后即可获得大量高纯度的成熟DC,经流式细胞仪检测,所得细胞中高表达共刺激分子和粘附分子,表现为成熟DC的特征.结论:利用多细胞因子组合可从外周血诱导大量成熟DC,为DC的临床应用提供依据.

Objective To induce highly purified mature dendritic cells(DC) from healthy human peripheral blood. Methods Plastic-adherent monocytes could be isolated from peripheral blood mononuclear cells by 2 hours incubation and then cultured in AIM-V with a serial cytokines. Results After 8～9 days incubation a largenumber of DC with high purity were generated. Tested by flow cytometer, these DCs highly expressed co-stimulating molecules and adherent molecules, showing the characteristics of mature DC. Conclusion Mature DC can be induced from peripheral blood with cytokines, which provides evidence for its clinical application.

参考文献：

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