高糖对肾小球系膜细胞c-fos表达与纤维结合蛋白分泌的影响-东南大学学报医学版

高糖对肾小球系膜细胞c-fos表达与纤维结合蛋白分泌的影响

单位：东南大学医学院,遗传与发育生物学系,江苏,南京,210009

关键词：葡萄糖肾小球膜细胞培养的糖尿病肾病基因 fos 基因表达纤连蛋白类/分析

分类号：R3

出版年·卷·期（页码）：2005·24·第一期（39-41）

摘要：

目的:研究高糖对人肾小球系膜细胞纤维结合蛋白(FN)分泌以及c-fos表达的影响,探讨高糖对人肾小球系膜细胞的作用.方法:采用人肾小球系膜细胞进行体外培养,高糖作为刺激因素,ELISA法测定培养上清中FN含量,免疫细胞化学方法检测c-fos表达情况.结果:细胞培养上清中FN含量,在对照组每孔中为(88.9±19.1)-ng,高糖组(156.0±40.9)-ng,甘露醇组(156.0±21.9)-ng,后两组显著高于对照组(均P<0.05);c-fos的光密值,在对照组中为0.14±0.02,高糖组0.28±0.03,甘露醇组0.28±0.04,后两组显著高于对照组(均P<0.01).结论:高糖可增加人肾小球系膜细胞c-fos的表达,促进FN的分泌.

Objective To investigate the production of fibronectin and expression of c-fos in mesangial cells cultured in high glucose and to explain the mechanism of diabetes nephropathy. Methods The human mesangial cells were cultured in high glucose. The production of fibronectin was analysed by ELISA and the expression of c-fos was assaied by immunocellchemistry. Result Compared with the contorol glucose,high glucose increased the expressoion of c-fos and the production of FN. Conclusion The expression of c-fos and the production of FN increases when treated with high glucose.

参考文献：

[1] TAKESHI I, MASAKAZU H, SHIRO M. Stretch-induced overproduction of fibronectin in mesangial cells is mediated by the activation of mitogen-activated protein kinase. 1999(3). doi:10.2337/diabetes.48.3.595
[2] LARSEN T H, SKAR R, FROTJOLD E K. Regional activation of the immediate-early response gene c-fos in infracted rat hearts. 1998(3). doi:10.1046/j.1365-2613.1998.00062.x
[3] 高景燕, 李新荣, 邓红. 高糖对人肾小球系膜细胞MAPK活性的影响. 东南大学学报(医学版)2003(3). doi:10.3969/j.issn.1671-6264.2003.06.002
[4] KIKKAWA R, KOYA D, HANEDA M. Progression of diabetic nephropathy, 2003(3)

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