SARS病毒蛋白保守性分析-东南大学学报医学版

SARS病毒蛋白保守性分析

单位：南京大学,医药生物技术国家重点实验室,江苏,南京,210093

分类号：R3

出版年·卷·期（页码）：2005·24·第一期（32-35）

摘要：

目的:寻找和确定SARS病毒中高保守的蛋白,以指导针对这些高保守蛋白为药物靶的药物分子设计.方法:采用比较基因组的方法和PSI-Blast搜索方法分别探讨SARS病毒不同蛋白在冠状病毒属内部及在正链RNA病毒内的保守情况.结果:确定了SARS非结构蛋白,特别是RdRp、Hel、nsP12、nsP13等蛋白在冠状病毒属内部表现很高的序列相似性,同时它们在正链RNA病毒范围内呈现不同程度的保守性.结论:主要参与病毒的复制、转录及后处理过程的非结构蛋白RdRp、Hel、nsP12、nsP13的高保守性,表明与病毒扩增相关的基因调控机制的保守是病毒进化的重要特征;同时这些非结构蛋白的高保守性及功能重要性表明它们更适合作为有效的药物靶分子.

Objective To identify the highly conserved proteins which will be potential targets in the design of the drugs.Methods Conservation status of SARS proteins among genus coronavirus and positivestrand viruses were investigated respectively by comparative genomic analysis and PSIBlast search in the database. Result We found that the nonstructural proteins, especially RdRp,Hel,nsP12 and nsP13, are evolutionally conserved. Conclusions Conservation status of nonstructural proteins reveals that conservation of replication, transcription and RNAprocessing machinery is a characteristic feature in the virus evolution. Also this suggests that the highly conserved proteins, RdRp,Hel,nsP12 and nsP13 will be potential drug targets. Our results provide theoretical guidance for the design of potential antiSARS drugs.

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