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应用原位杂交技术分析ZNF216基因在成年小鼠各主要组织中的表达模式
作者:顾香芳 甘光明 王芳 谢维 
单位:东南大学医学院,遗传与发育生物学系,江苏省基因诊断和治疗重点实验室,江苏,南京,210009
关键词:锌指/遗传学 ZNF216 原位杂交 荧光 基因表达 小鼠 
分类号:R3
出版年·卷·期(页码):2005·24·第一期(1-4)
摘要:

目的:获得ZNF216基因的表达模式.方法:体外转录方法合成地高辛标记的cRNA探针,利用原位杂交技术检测ZNF216基因在成年小鼠各主要组织中的表达模式.结果:在小鼠脑、心脏、骨骼肌和睾丸细胞内可检测到很强的ZNF216的杂交信号,在肾脏细胞内也可检测到杂交信号,但在脾脏和肝脏细胞内仅检测到少许杂交信号和未能检测到杂交信号.结论:ZNF216基因表达于几种组织细胞内,具有一定的组织特异性.

参考文献:

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