Objective To investigate the expression of v-raf murine sarcoma viral oncogene homolog B1(BRAF) gene in normal pancreas and pancreatic adenocarcinoma tissue.Methods Three cases of normal pancreas tissue and six cases of pancreatic adenocarcinoma were collected,which were confirmed by pathology.The RNA was extracted through the method of TRizol,purified by DNA enzyme Ⅰ and detected through violet-spectrophotometer,agarose gel(electrophoresis)(AGE) and Lab-on-chip methods.The expression of BRAF gene was detected by oligonucleotide microarray,which was comfirmed by reverse transcription polymerase chain reaction(RT-PCR).Results The ratio of OD_{260nm}/OD_{280nm} was between 1.8 and 2.0.The bands in 18()s and 28()s through AGE was clear,and the brightness ratio of the two bands(28()s and 18()s) was more than 2.0.The scales and situs of split-blip were normal through the detection of Lab-on-chip method.BRAF mRNA was overexpressed in pancreatic cancer tissue compared with normal pancreas.The scanning signal of microarray hybridization was clear,and the images had a lower background and higher signal-noise ratio.The expression of BRAF gene in pancreatic adenocarcinoma against normal pancreas was up-regulated(Cy3/Cy5>2.0)by Ratio analysis.Conclusion BRAF gene is overexpressed in pancreatic adenocarcinoma and may be involved in the pathogenesis of pancreatic cancer,but the role BRAF gene in the mechanisms of pancreatic cancer should be further investigated.