低频超声联合微泡剂对血管内皮细胞的生物学效应-东南大学学报医学版

低频超声联合微泡剂对血管内皮细胞的生物学效应

单位：东南大学附属中大医院,肿瘤科,江苏,南京,210009

分类号：R73, R-33

出版年·卷·期（页码）：2006·25·第五期（330-333）

摘要：

目的:研究低频超声联合微泡剂对血管内皮细胞VEC-304的作用,为超声治疗的临床应用提供实验依据.方法:将细胞分为空白对照(A)组;单纯微泡剂(B)组;单纯超声(C)组,因给予不同时间的超声辐照又分为C1组(60 s)、C2组(90 s)、C3组(120 s)和C4组(150 s);超声联合微泡剂(D)组,因加入微泡剂后立即给予不同时间的超声辐照又分为D1组(60 s)、D2组(90 s)、D3组(120 s)和D4组(150 s).MTT法观察细胞增殖的抑制作用,分光光度法检测细胞培养液中活性氧(ROS)和超氧化物歧化酶(SOD)活力的变化,流式细胞仪(FCM)检测细胞凋亡.结果:超声联合微泡剂能够显著抑制细胞增殖;C3、D3组培养液中ROS活力显著高于A、B两组(P＜0.01),SOD活力显著低于A、B两组(P＜0.01),且D3组效应比C3组更为明显;C3、D3组细胞凋亡率明显高于A、B组(P＜0.01),D3组高于C3组(P＜0.05).结论:低频超声联合微泡剂可能是通过增加细胞外ROS的活力来诱导对细胞的损伤和凋亡.

Objective To observe the bio-effects on VEC-304 treated with low-frequency ultrasound and micro bubble agent,and to offer the clinic application theory basis of ultrasound.Methods Cells were divided into four groups :control(A);pure microbubble agent(B);pure ultrasound(C),according to the exposure time,group C was subdivided into C_1(exposure 60()s),C_2(exposure 90()s),C_3(exposure 120()s) and C_4(exposure 150()s);low-frequency ultrasound combined with micro bubble agent(D),according to the exposure time,group D was subdivided into D_1(exposure 60()s),D_2(exposure 90 s),D_3(exposure 120 s) and D_4(exposure 150()s).The survival rate of VEC-304 was detected by MTT colormetric assay.Visible spectrophotometric was applied to determine ROS and SOD.Apoptosis was analyzed by flow cytometry after ultrasonic exposure.Results The proliferation of group D were obviously inhibited,which was detected by MTT colormetric assay.The yield of ROS of groups C_3 and D_3 was higher than groups A and B,respectively.The yield of SOD of groups C_3 and D_3 was lower than groups A and B,respectively.The apoptosis rates were found to reach(11.57±2.68)% in group C_3 and(21.15±4.68)% in group D_3 by FCM.Conclusion Low-frequency ultrasound combinding with microbubble agent can induce VEC-304 injury and apoptosis,which is probably due to the adding yield of ROS in cultured liquid.

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