Objective To study the effect of Jiangdan Decoction on expression of connective tissue growth factor(CTGF) in glomeruli of 5/6 nephrectomized rats with glomerulosclerosis,and investigate its mechanism in preventing and treating glomerulosclerosis.Methods The 5/6 nephrectomized rats with glomerulosclerosis were assigned to the control group,model group,losartan treatment group and Jiangdan Decoction treatment group.The levels of blood urea nitrogen,serum creatinine and cholesterol were detected at the 12th weekend after operation.Renal pathology changes were observed at the 12th week by light microscope and electron microscope.Immunohischemistry was used to examine the expression of CTGF in glomeruli.Results The levels of blood urea nitrogen,serum creatinine and cholestrol in the Jiangdan Decoction group and losartan group were significantly lower than in the model group(P<0.01),every target was similar in the two treatment groups(P>0.05).The renal pathology examination showed that the degree of glomerular proliferation and glomerulosclerosis is significantly attenuated in the two treatment groups.Immunohistochemistry staining indicated that the expression of CTGF in glomeruli of the two treatment groups declined,respectively(P<(0.01)),this target was similar in the two groups(P>0.05).Conclusion Jiangdan Decoction can significantly inhibit the over-expression of CTGF in glomeruli of 5/6 nephrectomized rats with glomerulosclerosis,its mechanism in preventing and treating glomerulosclerosis is found relating to the inhibitory effect on the over-expression of CTGF. |
