哮喘患者诱导痰上清液MIP-1α、MIF、MCP-1水平测定-东南大学学报医学版

哮喘患者诱导痰上清液MIP-1α、MIF、MCP-1水平测定

单位：南京大学医学院附属鼓楼医院,呼吸科,江苏,南京,210008

关键词：哮喘诱导痰肺泡巨噬细胞炎性蛋白-1α 肺泡巨噬细胞移动抑制因子单核细胞趋化蛋白-1

分类号：R562.25

出版年·卷·期（页码）：2006·25·第四期（268-271）

摘要：

目的:通过测定哮喘患者诱导痰上清液肺泡巨噬细胞炎性蛋白-1α(MIP-1α)、肺泡巨噬细胞移动抑制因子(MIF)、单核细胞趋化蛋白-1(MCP-1)的水平,探讨三者在哮喘发病中的作用.方法:轻、中度哮喘患者14例,正常人12例,用高渗盐水进行痰诱导,痰液经解粘、离心后,对沉淀细胞分类计数,使用ELISA测定诱导痰上清液中MIP-lα、MIF、MCP-1浓度.结果:哮喘组诱导痰嗜酸粒细胞、淋巴细胞相对计数均高于正常组(分别为7.7%±1.4%、0.7%±0.8%,P<0.01;4.2%±1.0%、2.1±0.8%,P<0.01),而哮喘组诱导痰肺泡巨噬细胞相对计数构成比(57.8%±3.7%)明显低于正常组(65.9%±3.6%,P<0.01).哮喘患者诱导痰上清液MIP-1α质量浓度[(36.6±4.8)pg·ml-1]明显高于正常人[(32.6±1.7)pg·ml-1,P<0.05],两组诱导痰上清液MIF、MCP-1浓度无显著差异.哮喘患者诱导痰上清液MIF与嗜酸粒细胞相对计数呈正相关(r=0.53,P<0.05),与肺泡巨噬细胞、淋巴细胞相对计数无相关关系;哮喘患者诱导痰上清液中MIP-1α、MCP-1与肺泡巨噬细胞、嗜酸粒细胞和淋巴细胞相对计数均无相关关系.结论:MIP-1α可能参与哮喘的发病,嗜酸粒细胞可能通过释放MIF参与哮喘气道炎症.

Objective To determine the concentration of macrophage inflammatory protein-1 alpha(MIP-1α),macrophage migration inhibitory factor(MIF)and monocyte chemoattractant protein-1(MCP-1)in induced sputum supernate from asthmatac patients,and explore the roles of MIP-1α,MIF and MCP-1 in asthmatics.Methods Induced sputum samples were collected from 14 mild and moderate asthmatic patients and 12 healthy individuals.The differential counts of inflammatory cells were studied in induced sputum.The concentration of MIP-1α,MIF and MCP-1 in induced sputum supernate was measured by sandwich enzyme linked immunosorbent assay(ELISA).Results The percentages of eosinophils and lymphocytes in induced sputum supernate from asthmatic patients were higher than those from healthy individuals(P<0.01).The percentage of macrophages from asthmatic patients was lower than that from healthy individuals((P<0.05).)The concentration of MIP-1α in asthmatics was increased compared with that in healthy individuals((P<0.05).)The concentration of MIF and MCP-1α had no difference between asthmatics and healthy individuals.The concentration of MIF in induced sputum supernate had close relationship to percentage of the eosinophils,but did not correlate to macrophages and lymphocytes.There was not any relationship between MIP-1α or MCP-1 and macrophages,eosinophils or lymphocytes.Conclusions The eosinophils and lymphocytes are important inflammatory cells in the pothgenesis of asthma.MIP-1α may play a role in asthmatics.Eosinophils may take part in the airway inflammation by producing MIF.

参考文献：

[1] YAMAGUCHI E, NISHIHIRA J, SHIMIZU T. Macrophage migration inhibitory factor(MIF) in bronchial asthma. 2003. doi:10.1046/j.1365-2222.2000.00888.x
[2] ALAM R, YORK J, BOYARS M. Increased MCP-1,RANTES,and mip-1 alpha in bronchoalveolar lavage fluid of allerge asthmatic patients. 1996(4)
[3] 肖建武. 支气管哮喘患儿血清单核细胞趋化蛋白-1浓度的初步研究. 中华医学丛刊2002(8)
[4] LOUIS R, SELE J, HENKET M. Sputum eosinophil count in a large population of patients with mild to moderate steroid-naive asthma:distribmion and relationship with methacholine bronchial hyper responsiveness. 2002. doi:10.1034/j.1398-9995.2002.23608.x
[5] SHIOTA Y, MATSUMOTO H, HIYAMA J. Flow cytometric analysis of lymphocytes and lymphocyte subpopulations in induced sputum from patients with asthma, 2002
[6] 李昌崇, 张维溪, 陈小芳. 巨噬细胞炎性蛋白lα及其mRNA在哮喘小鼠气道炎症中的作用. 中华儿科杂志2004(2)
[7] CRUIKSHANK W W, LONG A, TARPY R E. Early identification of interleeukin-16 (lymphocyte chemoattractant factor)and macrophage in flammatory protein l alpha(MIP-1 alpha)in bronchoalveolar lavage fluid of antigen challenged asthmatics, 1995
[8] SZALAI C, KOZMA G T, NAGY A. Polymorphism in the gene regulatory region of MCP-1 is associated-with asthma susceptibility and severity. 2001(3). doi:10.1067/mai.2001.117930
[9] CONTI P, DiGIOACCHINO M. MCP-1 and RANTES are mediators of acute and chronic in flammation, 2001

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录