Objective To explore whether GM-CSF gene immunization could stimulate potent immune response in tumor-bearing mice and lay a foundation for GM-CSF gene treatment tumor.Methods After establishing tumor animal model by subcutaneously inoculating SP2/0 into Balb/c mice,the blank or the recombinant plasmid of pc-mGM-CSF was respectively injected into tumor-bearing mice subcutaneously or intramuscularly for 4 times totalling up to 400()μg for each mouse.Eight weeks later, tumor formation rate,tumor weight,lymphocytes infiltration in tumor tissue and the levels of IFN-γ,IL-2 in the serum of all mice were detected and the activities of CTL and NK were examined with MTT assay.Results After tumor-bearing mice were treated with the GM-CSF recombinant for eight weeks,tumor weight [(0.880±0.405)g] was reduced in mice injected subcutaneously with the GM-CSF recombinant compared with control mice [(1.548±0.221) g,P <0.01],and the tumor weight [(0.378±0.411) g] was remarkably reduced in mice intramuscularly injected with the GM-CSF recombinant compared with control mice [(1.554±0.249) g,P <0.001].The phenomena of lymphocyte infiluration in tumor tissues was very apparent in the mice intramuscularly injected with the GM-CSF recombinant.In addition,the levels of IFN-γ,IL-2 and the activities of CTL and NK cells in tumor-bearing mice injected intramuscularly with the GM-CSF recombinant were higher than those of other groups.Conclusion GM-CSF gene immunization can induce antitumor effects and may be a novel approach to tumor therapy in future. |
