甲泼尼龙和神经节苷脂对大鼠损伤脊髓CD95表达的影响-东南大学学报医学版

甲泼尼龙和神经节苷脂对大鼠损伤脊髓CD95表达的影响

单位：1.东南大学医学院附属徐州医院,骨科,江苏,徐州,221009； 2.东南大学附属中大医院,骨科,江苏,南京,210009

分类号：R651.2

出版年·卷·期（页码）：2006·25·第三期（191-196）

摘要：

目的:研究甲泼尼龙(MP)加神经节苷脂(GM1)对大鼠脊髓损伤(SCI)的疗效.方法:采用Allen's法制备SCI大鼠模型.将模型大鼠随机分为4组,A组为生理盐水对照组,B组为MP治疗组,C组为GM1治疗组,D组为MP加GM1联合治疗组.大鼠存活不同时间后分别行行为学观察再处死,取伤段脊髓作石蜡切片,行HE染色和CD95单克隆抗体免疫组化检测.结果:HE染色显示脊髓组织病理学改变B、C、D组明显轻于A组,而D组较B、C组轻.伤后2周行为学指标优差依次为D-C-B-A(P＜0.05).免疫组化检测表明,A组脊髓灰质CD95免疫染色阳性细胞数在8 h达到高峰,在白质中48 h时达到高峰,而D组虽有高峰,但明显少于A组(P＜0.01),D组也少于B、C组(P＜0.01).结论:MP与GM1联合应用可阻断神经细胞的凋亡,对损伤的脊髓组织有良好的保护作用并可促进神经功能的恢复.

Objective To investigate the curative effect of methylprednisolone(MP)and ganglioside(GM_1) on rats’ spinal cord injury.(Methods)Preparing rats with spinal cord injury by resorting to Allen’s mode and then dividing them into four groups at random: group A(control),group B(with MP),group C(with GM_1) and Group D((with) MP and GM_1).After different periods of surviving time,behavior of different rat was observed respectively and detected by Hemetoxylin staining and CD95 monoclonal antibody immunohistochemistry.Results In injured spinal cord,the pathological changes of group B,C and D were less obvious than that of group A,while group D was less obvious than group B and C.After two weeks of the injury,the order from superiority to inferiority of the behavioral index was D-C-B-A(P<0.05,respectively).The immunohistochemical studies showed that in group A,the number of the CD95 immunostained positive cells in gray matter peaked after 8 hours,and in white matter it peaked after 48 hours.However,although group D had its peak,it was obviously less than group A(P<0.01),and also less than group B and C((P<0.01)). Conclusion The combined application of MP and GM_1 can stop the apoptosis of nerve cells,provide adequate protection for injured spinal cord and enhance the recovery of nerve functions.

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