Objective To investigate the effect of MnCl_2(0,150,300μmol·L^{-1})on the antitumor activity of human peripheral blood dendritic cells(DC).Methods Factorial design and orthogonal design were adopted to analysis the effect of MnCl_2(0,150,300μmol·L^{-1}),DC(2×10^3,1×10^4,2×10^4ml^{-1}),E/T(5∶1,10∶1,20∶1)on the antitumor activity of DC;flow cytometry was applied to examine the expression of HLA-DR on the groups of MnCl_2-stimulated DC and the non-MnCl_2stimulated DC.The effect of the best stimulated-DC by the MnCl_2(300μmol·L^{-1})on the cytotoxicity of T cells in SCID mice attacked by hepatic tumor cells(Huh_7)was studied.Results These three factors all had a remarkable effect on the antitumor activity of DC.Both the expression of HLA-DR and the positive percentage of HLA-DR of MnCl_2-stimulated DC were much higher than that of non-MnCl_2-stimulated DC.The occurrence rate and the general volume of tumor in the SCID group injected with MnCl_2-stimulated DC were remarkably less than that of the other group injected with non-MnCl_2-stimulated DC.Conclusion MnCl_2 can remarkably enhance the expression and positive percentage of HLA-DR in DC in vitro.MnCl_2-stimulated DC can remarkably augment the cytotoxicity of T cells and remarkably suppress the growth of tumor(Huh_7) in SCID. |
