骨肉瘤组织芯片的制作及其免疫组织化学应用-东南大学学报医学版

骨肉瘤组织芯片的制作及其免疫组织化学应用

单位：南京大学医学院附属鼓楼医院,病理科,江苏,南京,210008

分类号：R738.1

出版年·卷·期（页码）：2006·25·第二期（104-107）

摘要：

目的:研究骨肉瘤组织芯片的制备方法及其免疫组织化学(免疫组化)应用.方法:选取脱钙骨肉瘤石蜡包埋标本36例,应用微阵列打孔仪制备组织芯片,并进行Ki67和CD34免疫组化标记.结果:制备的骨肉瘤组织芯片组织完整、排列整齐,无掉片脱片现象,免疫组化标记结果良好,无边缘效应.结论:可以联合应用组织芯片技术和免疫组化技术对脱钙骨肉瘤进行研究.

Objective To study how to develop the tissue microarray of osteosarcoma and the application of immunohistochemical staining on the tissue microarray.Methods Paraffin-embedded tissue of 36 cases of osteosarcoma were selected to develop the tissue microarray with the manual tissue arrayer;and immunohistochemical staining labelled with Ki67 and CD34 were performed on the tissue microarray of osteosarcoma.Results The developed tissue microarray of osteosarcoma were arrayed integrally and trimly,none of the tissue microarrays on the section was lost,and the immunohistochemical staining was excellent,without marginal or non-specific staining.Conclusions Tissue microarray can be used to study osteosarcoma with immunohistochemistry.

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