褪黑素、维生素E和还原型谷胱甘肽对四氧嘧啶糖尿病大鼠氧化应激影响的比较研究-东南大学学报医学版

褪黑素、维生素E和还原型谷胱甘肽对四氧嘧啶糖尿病大鼠氧化应激影响的比较研究

单位：1.东南大学附属中大医院,内分泌科,江苏,南京,210009； 2.首都医科大学附属天坛医院,内分泌科,北京,100050

分类号：R587.02

出版年·卷·期（页码）：2006·25·第二期（88-92）

摘要：

目的:探讨褪黑素(MLT)、维生素E(VE)和还原型谷胱甘肽(GSH)对糖尿病大鼠肝、肾和胰组织氧化应激的影响.方法:对四氧嘧啶诱导的糖尿病大鼠分别给予MLT、VE和GSH治疗14 d后,观察各组大鼠肝、肾和胰组织中丙二醛(MDA)、一氧化氮(NO)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px )活性的变化.结果:与正常对照组大鼠比较,糖尿病大鼠肝、肾和胰组织MDA、NO含量显著升高,GSH-Px活性上升,而SOD活性显著降低.给予MLT、VE和GSH治疗可明显降低肝、肾、胰组织中MDA和NO含量,使升高的GSH-Px活性显著下降,并显著增强SOD活性,但GSH对肝脏SOD无明显影响.3种抗氧化剂相比,MLT在大鼠肾脏和胰腺组织的抗氧化能力明显强于VE和GSH,而在大鼠肝脏组织VE的抗氧化能力优于MLT和GSH.结论:MLT可通过增强糖尿病大鼠的抗氧化能力和抑制氧化应激反应,在糖尿病及其并发症发生、发展中具有一定的保护作用,并且MLT的抗氧化能力明显强于VE和GSH.

Objective To investigate the effects of melatonin(MLT),vitamin E(VE) and glutathione(GSH) on oxidative stress in liver,kidney and pancreas tissue of diabetic rats.Methods Alloxan(200?mg·kg^{-1} i.p.)-induced diabetic rats were treated with MLT(2?mg·kg^{-1} i.p.) or VE(100?mg·kg^{-1} i.p.) or GSH(100?mg·kg^{-1} i.p.) for 14 days.Lipid peroxidation(MDA),nitric oxide(NO) levels and superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) activity in liver,kidney and pancreas tissue were measured.Results MDA and NO levels and GSH-Px activity were significantly increased and SOD activity was significantly decreased in liver,kidney and pancreas in alloxan-induced diabetic rats compared with normal rats.In rats treated with MLT,VE or GSH,MDA and NO levels were decreased significantly,and elevated GSH-Px activity reversed significantly,while SOD activity increased significantly,but GSH had no effect on liver SOD activity.The antioxidant effects of MLT were greater than that of VE and GSH in kidney and pancreas whereas VE was more potent than MLT and GSH in liver.Conclusion MLT prevents the development and progression of diabetes,as well as its complications by enhancing antioxidative function and reducing oxidative stress in diabetic rats. Moreover,MLT may be a more powerful antioxidant than VE and GSH.

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