热应激反应对中性粒细胞NADPH氧化酶活性及呼吸爆发功能的影响-东南大学学报医学版

热应激反应对中性粒细胞NADPH氧化酶活性及呼吸爆发功能的影响

单位：1.上海市第八人民医院,康复科,上海,200235； 2.东南大学附属中大医院,康复科,江苏,南京,210009

分类号：R594.1

出版年·卷·期（页码）：2007·26·第六期（430-433）

摘要：

目的:观察体外诱导热应激反应对中性粒细胞凋亡及呼吸爆发功能的影响.方法:将健康人中性粒细胞分成3份,1份作为对照组,其余2份应用热休克或氯化镉诱导热应激反应后与培养液共同孵育,并分别作为热休克组、氯化镉组.各组(n=18)于热应激反应诱导后0、2、3、4、6 h分别测定中性粒细胞热休克蛋白(HSP)70的表达、NADPH氧化酶活性及呼吸爆发功能.结果:热休克与氯化镉均可诱导热应激反应,细胞内HSP70表达增加;热应激反应抑制中性粒细胞NADPH氧化酶活性并降低其呼吸爆发功能.结论:热应激反应通过抑制中性粒细胞NADPH氧化酶活性减少O2-释放,提示热应激反应可能参与机体的抗炎作用机制.

Objective To explore the effect of heat stress response on the activity of NADPH oxidase and respiratory burst in neutrophils.Methods Neutrophils from each of 18 healthy volunteers were divided into control group,heat shock group that were induced by heat shock or cadmium for heat stress response and cadmium chloride group respectively.The neutrophils were incubated in culture medium.At 0,2,3,4,6 h following heat stress induction,the heat shock protein(HSP70) expression and the respiratory burst activity were detected in the neutrophils respectively,and the activity of NADPH oxidase was observed at 0,3 h after heat stress induction.Results Heat stress response was successfully induced in the heat shock and cadmium chloride group,a progressive increase in HSP70 expression in neutrophils was detected.The neutophil respiratory burst activity decreased after heat stress response,and the NADPH oxidase activity was inhibited in the cells subjected to the stress.Conclusion Heat stress response can inhibit the NADPH oxidase activity and decrease respiratory burst activity in neutrophils,indicating that heat stress response may play a role in anti-inflammatory roles.

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