大鼠脑缺血后皮质PSD-93基因表达的动态变化-东南大学学报医学版

大鼠脑缺血后皮质PSD-93基因表达的动态变化

单位：1.南京大学医学院附属鼓楼医院,神经内科,江苏,南京,210093;南京大学,医药生物技术国家重点实验室,江苏,南京,210008； 2.南京大学,医药生物技术国家重点实验室,江苏,南京,210008

分类号：R-33, Q786, R364.12

出版年·卷·期（页码）：2007·26·第六期（403-406）

摘要：

目的:观察突触后密度(PSD)-93基因在大鼠局灶性脑缺血再灌注皮质表达的变化,探讨其在缺血再灌注损伤中的病理作用.方法:线栓法建立大鼠大脑中动脉闭塞再灌注模型,缺血2 h后,分别再灌注6、12和24 h,应用RT-PCR、Western bolt 技术检测缺血再灌注后皮质PSD-93基因mRNA和蛋白的表达.结果:缺血再灌注的非缺血侧大脑皮质PSD-93 mRNA和蛋白的表达与对照组相比均无明显变化;缺血侧皮质PSD-93 mRNA表达明显高于未缺血侧,12、24 h组与未缺血侧比较具有显著性差异(均P＜0.05),并呈时间依赖性;再灌注6 h后PSD-93蛋白表达升高,再灌注12 h达高峰,12 h组与未缺血侧比较具有显著性差异(P＜0.05).结论:脑缺血再灌注后皮质PSD-93基因表达增高,推测PSD-93参与介导缺血性脑损伤.

ObjectiveTo investigate the expression and pathological function of postsynaptic density-93(PSD-93) after rat focal ischemic middle cerebral artery occlusion/reperfusion(MCAO).MethodsAfter the right middle cerebral artery occluded for 2 hours,the rats were reperfused for 6,12 and 24 h before decapitation.PSD-93 gene and protein in the cortex were measured by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.ResultsThere were no differences of the expression of PSD-93 between the sham operation-group and the non-ischemic sides;PSD-93 mRNA gradually increased,and the expressions on the ischemic sides were higher than that on the non-ischemic sides.The expressions of 12,24 h groups were increased significantly(P<0.05) in a time-dependent manner;PSD-93 protein expression level increased at 6 h and reached the peak at 12 h,showing a significant difference at 12 h(P<0.05).ConclusionsThese results indicate that PSD-93 gene expression may be up-regulated after reperfusion following focal cerebral ischemia.And this change may be involved in the pathogenesis of focal cerebral ischemia.

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