氨磷汀对化疗药物抗宫颈癌HeLa细胞的影响-东南大学学报医学版

氨磷汀对化疗药物抗宫颈癌HeLa细胞的影响

单位：1.东南大学医学院附属南京第二医院,妇产科,江苏,南京,210009； 2.东南大学临床医学院,中心实验室,江苏,南京,210009

分类号：R453, R-33

出版年·卷·期（页码）：2007·26·第四期（316-317）

摘要：

目的:评价氨磷汀(amifostine)对不同化疗药物抗宫颈癌HeLa细胞的影响.方法:用MTT法分别测定顺铂、长春新碱、依托泊甙和丝裂霉素对体外培养的HeLa肿瘤细胞系的抑制作用,实验组加化疗药前30 min经600 mg·L-1氨磷汀处理,对照组则只用化疗药物处理,余同实验组,培养后比较两组细胞增殖的抑制率.结果:用氨磷汀在体外加药前30 min加入HeLa肿瘤细胞的实验组,与对照组比较,两组肿瘤细胞抑制率无显著性差异(P＞0.05).结论:氨磷汀不影响顺铂、依托泊甙、丝裂霉素和长春新碱对HeLa肿瘤细胞的抑制作用.

Objective To evaluate the therapeutic effect of amifostine on different chemistry anticese HeLa cell in vitro.Method MTT was used to investigate cell inhibition effects of amifotine for the HeLa cell received chemotherapy of DDP,MMC,VCR,VP-16 respectively.The cell lines were divided into two groups:amifostine groups and control groups.The control groups only received DDP,MMC,VCR,or VP-16,respectively.The cell lines in amifostine groups received 600 mg·L-1 amifostine 30 minutes before chemotherapy.The inhibitory rate of two groups was compared.Result There was no significant difference of the inhibitory rate between amifostine grpups and control groups(P>0.05).Conclusion Amifotine has no effect on the chemotherapy of DDP,MMC,VCR and VP-16 on HeLa cell.

参考文献：

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