目的:建立骨髓间充质干细胞与髓核细胞共培养的模型,研究间充质干细胞对体外培养的髓核细胞活性的影响.方法:通过transwell插入式培养皿构建间充质干细胞-髓核细胞共培养模型,观察共培养条件下髓核细胞形态变化,计数细胞增殖,ELISA方法检测蛋白多糖含量变化.结果:经过8 d共培养,实验组中的髓核细胞计数较对照组增加明显(P＜0.01),培养上清液中蛋白多糖合成较对照组明显提高(P＜0.01).结论:成功建立共培养模型,与间充质干细胞共培养后髓核细胞活性上调.

[1] NISHIMURA K, MOCHIDA J. Percutaneous reinsertion of the nucleus pulposus. 1998. doi:10.1097/00007632-199807150-00006
[2] ICHIMURA K, TSUJI H, MAKIYAMA N. Cell culture of the interveaebral disc of rats:factors influencing culture,proteoglycan,conagen,and deoxyribonucleic acid synthesis, 1991(4)
[3] AQUIAR D J, JOHNSON S L, OEGEMA T R. Notochordal cells interact with nucleus pulposus cells:regulation of proteoglycan synthesis. 1999(1). doi:10.1006/excr.1998.4287
[4] OKUMA M, MOCHIDA J, NISHIMURA K. Reinsertion of stimulated nucleus pulposus cells retards intervertebral disc degeneration:an in vitro and in vivo experimental study. 2000(6). doi:10.1002/jor.1100180620
[5] MINGES-WOLS H A, UNDERHILL G H, KANSAS G s. The role of bone marrow-derived stromal cells in the maintenance of plasma cell longevity. 2002
[6] MIZUGUCHI T, PLAM K, HUI T. Effects of bone marrow stromal cells on the structural and functional polarity of primary rat heaptocytes, 2002
[7] 李秀森, 郭子宽, 杨靖清. 骨髓间充质干细胞的生物学特性. 解放军医学杂志2000(5)
[8] THOMPSON J P, Jr OEGEMA T R, BRADFORD D S. Stimulation of mature canine intervetebral disc by growth factors. 1991. doi:10.1097/00007632-199103000-00001
[9] YAMAMOTO Y, MOCHIDA J, SAKAI D. Upregulation of the viability of nucleus pulposus ceils by bone marrow-derived stromal cells:significance of direct cell-to-cell contact in cocuhure system. 2004. doi:10.1097/01.BRS.0000131416.90906.20
[10] BARTHOLOMEW A, STURGEON C, SIATSKAS M. Mesenchymal stem ceils suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo. 2002(1). doi:10.1016/S0301-472X(1)00769-X
[11] GRUBER H, HANLEY E. Human disc cells in monolayer vs 3D culture:cell shape,division and matrix formation. 2000. doi:10.1186/1471-2474-1-1
[12] 鲁玉来, 蔡钦林. 腰椎问盘突出症, 2001