重组人生长激素在体外对人胃癌细胞作用的研究-东南大学学报医学版

重组人生长激素在体外对人胃癌细胞作用的研究

单位：东南大学,临床医学院,江苏,南京,210009

分类号：R735.2, R969

出版年·卷·期（页码）：2007·26·第三期（206-209）

摘要：

目的:研究重组人生长激素(rhGH)在体外对胃癌细胞生长的影响.方法:设对照组、rhGH组、5-氟尿嘧啶(5-FU)组和rhGH加5-FU 组共4组,依rhGH浓度不同,rhGH和rhGH加5-FU组又各分4个亚组.利用体外细胞培养、MTT比色、流式细胞仪及免疫细胞化学等方法,测定不同浓度的rhGH对人胃癌细胞株MGC803肿瘤抑制率、细胞周期、细胞增殖指数(PI)及核增殖抗原Ki-67表达的影响.结果:rhGH在体外无明显促进MGC803细胞分裂增殖作用;对照组与各rhGH组比较,肿瘤抑制率、PI、Ki-67表达强度差异均无显著性(P＞0.05);rhGH各亚组间比较差异亦无显著性;5-FU组与rhGH组及对照组比较,各rhGH加5-FU组与rhGH组及对照组比较细胞抑制率增加,阻滞于G0～G1期的细胞数增加,S期细胞明显减少,PI明显降低(P＜0.05),Ki-67表达强度明显下降.结论:重组人生长激素在体外不会促进胃癌细胞的增殖.

Objective To evaluate the effects of recombinant human growth hormone(rhGH) on growth of human gastric cancer cell in vitro.Methods Four groups were respectively assigned as control group,rhGH group,5-FU group and rhGH+5-FU group.Following cell culture,MTT assay,flow cytometry and immunocytochemistry,effects of differenct concentrations of rhGH on cell inhibitory rate,cell cycle proliferation index(PI) and Ki-67 express intensity of human gastric cancer line MGC803 were studied.Results In vitro the distinctly accelerated effects of rhGH on multiplication of MGC803 cell line were not found.There was no statistically significant difference of cell inhibitory rate,PI and Ki-67 expressing intensity between rhGH group and control group or between rhGH+5-FU group and 5-FU group(P>0.05).Cell inhibitory rate and cell arrested in G0/G1 phase were obviously increased,while the cells in S phase and PI greatly decreased in rhGH+5-FU group as compared with control group or rhGH group,also Ki-67 expressing intensity greatly decreased(P<0.05).Conclusion In vitro rhGH does not accelerate the proliferation of human gastric cancer cells.

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