5-FU对SW480细胞Musashi-1基因表达的影响-东南大学学报医学版

5-FU对SW480细胞Musashi-1基因表达的影响

单位：东南大学附属中大医院,肿瘤中心,江苏,南京,210009

关键词：结直肠癌细胞株SW480 Musashi-1基因肿瘤干细胞 5-氟尿嘧啶

分类号：R735.3, Q786, R979.1

出版年·卷·期（页码）：2007·26·第三期（198-201）

摘要：

目的:观察5-氟尿嘧啶(5-FU)对人结直肠癌细胞株SW480的作用与Musashi-1基因表达变化的关系.方法:用MTT法检测不同时间5-FU对SW480细胞增殖的影响,流式细胞术检测侧群(SP)细胞和CD34+CD44+细胞比例,RT-PCR半定量检测Musashi-1基因表达.结果:5-FU作用于SW480细胞24、48、72 h的半数抑制率依次为32.12、16.2和6.8 mg·L-1,SP细胞比例、CD34+CD44+细胞以及Musashi-1 mRNA表达在5-FU作用后均增加.结论:正常肠道干细胞标记物Musashi-1有可能成为结直肠癌干细胞样肿瘤细胞特异性标记,CD34、CD44可能具有类似的潜力.

Objective To investigate the effect of 5FU on colorectal cancer cell lines SW480 and clarify the role of Musashi-1 in the effect.Methods IC50(50% inhibiting concentration)of 5-FU was measured by MTT assay.The proportion of SP cells and CD34+CD44+cells were obtained by flow cytometry(FCM).The expressions of Musashi-1 mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results The IC50 of 5-FU at different time point as 24,48 and 72 h was 32.12、16.2 and 6.8 mg·L-1 respectively.Compared with normal control group,the proportion of SP cells was increased in experimental groups,as well as the proportion of CD34+CD44+ cells and expressions of Musashi-1 mRNA.Conclusion Musashi-1 may become one of markers for cancer stem cells in colorectal cancer cells.CD34 and CD44 have silimar potentials.

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