口服耐受后过继转输动物模型的建立方法-东南大学学报医学版

口服耐受后过继转输动物模型的建立方法

作者：邓晓静¹ 陈宝安² 丁家华² 孙雪梅³ 董伟民² 毕延智² 张琰² 赵刚² 高冲² 孙耘玉² 王骏² 程坚² 马燕² 宋慧慧<

单位：1.东南大学,临床医学院,江苏,南京,210009； 2.东南大学附属中大医院,血液科,江苏,南京,210009； 3.南京大学医学院附属鼓楼医院,血液科,江苏,南京,210009； 4.德国乌尔姆大学医学院内科Ⅲ区,德国,乌尔姆,D89081

关键词：口服耐受过继转输移植物抗宿主病动物模型小鼠

分类号：R457.7

出版年·卷·期（页码）：2007·26·第三期（161-166）

摘要：

目的:探讨经口服受鼠脾淋巴细胞诱导耐受的供鼠淋巴细胞输注是否能减轻移植物抗宿主病(GVHD).方法:以雄性DBA-2小鼠(H-2d)为供者,分别用BALB/C鼠脾淋巴细胞、DBA-2鼠脾淋巴细胞、牛血清蛋白、普通饲料隔天喂养,单向混合淋巴细胞反应(MLR)评估诱导耐受状态.以雌性BALB/C小鼠(H-2d)为受者,给予6.0 Gy 60Coγ射线全身照射,尾静脉输注来自于DBA-2小鼠的2×107脾淋巴细胞.结果:耐受供鼠cpm值低于非耐受组,接受已耐受供鼠脾淋巴细胞输注的受鼠,发生GVHD较其他组轻.结论:经口服耐受的过继转输动物模型的实验方法有效可行.

Objective To investigate whether oral antigens have the effects of amelioration of graft-versus-host disease(GVHD) in mice in donors toward recipient splenocytes.Methods Male(DBA2)donor mice(H-2d) were fed with BALB/C splenocytes,DBA-2 splenocytes,bovine serum albumin,or regular chow,every other day.Induction of tolerance was assessed by a mixed lymphocyte reaction(MLR).Female(BALB/C) mice(H-2d) as recipients received total body irradiation(TBI) of 6.0 Gy(60Co γ-ray)followed by injection of 2×107splenocytes supplied by DBA-2.Results GVHD was significantly ameliorated in mice as recipients transplanted from tolerized dornors compared with control animals.Conclusion The establishment of adoptive transfer animal model with oral tolerance is feasible and effective.

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