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盐酸罗格列酮对糖基化终产物诱导心肌成纤维细胞增殖及分泌NO的影响
作者：李洁 刘乃丰 魏芹 
单位：东南大学附属中大医院,老年科,江苏,南京,210009
关键词：盐酸罗格列酮 糖基化终产物 心肌成纤维细胞 一氧化氮 
分类号：R329.28, R-33
出版年·卷·期（页码）：2007·26·第二期（128-130）
摘要：

目的:探讨盐酸罗格列酮对糖基化终产物(AGEs)诱导新生大鼠心肌成纤维细胞(CFs)增殖及分泌一氧化氮(NO)的影响.方法:采用胰酶消化法和差速贴壁分离法获取CFs,应用MTT法、流式细胞术(FCM)分别观察不同浓度盐酸罗格列酮对AGEs作用下CFs的细胞增殖、细胞周期的影响;硝酸还原酶法分别测定不同条件下CFs培养液中的NO水平.结果:AGEs对CFs的增殖有显著的促进作用;不同浓度的盐酸罗格列酮干预后,与AGEs组对比A值均明显下降(P＜0.05);S期及G2/M期细胞百分值明显低于AGEs组,而G0/G1 期的细胞百分值增高(P＜0.05),且随着浓度的增加,抑制细胞增殖作用越显著.AGEs抑制CFs分泌NO,盐酸罗格列酮可阻断AGEs的上述作用,并呈一定的浓度依赖关系(P＜0.01).结论:在一定浓度范围内,盐酸罗格列酮呈剂量依赖性地抑制AGEs诱导的大鼠CFs增殖,并阻断AGEs抑制CFs分泌NO.

Objective To investigate the effect of rosiglitazone(RGZ) on the proliferation and NO secretion of neogenetic rat’s cardiac fibroblasts(CFs)induced by advanced glycosylation end products(AGEs).Methods In vitro,the acquired rat CFs were incubated with AGEs(100mg·L-1) in the presence of different dose of RGZ.The proliferation of CFs was determined by MTT methods.The cell cycle of CFs was observed by flow cytometric analysis.The level of NO was measured by the method of nitrate reductase.Results AGEs significantly accelerated the CFs proliferation.Compared with AGEs group,A value was significantly reduced in the different dose RGZ groups(P<0.05).The percentages of cells in DNA synthetic(S) and mitotic phase(G2/M) were lower than that of AGEs group while the percentage of cells in G0/G1 was increased(P<0.05).With the increasing dose of RGZ,the inhibition became more significant.The results showed that the AGEs inhibited the NO secretion of CFs.It could be blocked by RGZ in a dose-related manner.Conclusion RGZ can inhibit the proliferation of CFs induced by AGEs with the dose-dependence in certain limits,and it can block the inhibition of AGEs on NO secretion of CFs.
参考文献：

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