参麦注射液对新生大鼠缺氧缺血性脑损伤后学习及记忆能力的改善作用-东南大学学报医学版

参麦注射液对新生大鼠缺氧缺血性脑损伤后学习及记忆能力的改善作用

单位：1.东南大学附属中大医院,儿科,江苏,南京,210009； 2.东南大学,临床医学院,江苏,南京,210009； 3.南京市妇幼保健医院,妇产科,江苏,南京,210004

分类号：R722.1, R-33

出版年·卷·期（页码）：2007·26·第二期（90-94）

摘要：

目的:探讨中药参麦注射液对新生大鼠缺氧缺血性脑损伤(HIBD)后学习和记忆能力的改善作用.方法:7日龄SD大鼠随机分为参麦注射液干预组、胞二磷胆碱干预组、生理盐水干预组、假手术组和正常对照组5组.前3组均在成模后当日开始每组每只分别给予参麦注射液、胞二磷胆碱和生理盐水腹腔注射干预,1次·d-1,连续14d.其余两组不做处理.应用Y-型电迷宫对各组第60及90日龄实验大鼠测试学习能力,并于24h后(第61、91日龄)复测,检测各实验大鼠24h记忆保持能力.结果:经过参麦注射液和胞二磷胆碱分别干预后,其学习能力及24h记忆能力与生理盐水干预组比较有明显改善(P＜0.05),参麦注射液组与胞二磷胆碱组之间也有统计学差异(P＜0.05).结论:参麦注射液和胞二磷胆碱均可改善大鼠HIBD后的学习和记忆能力,前者优于后者.

Objective To investigate the effect of Shenmai injection on learning and remembering ability of neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods Seven-day old Sprague-Dawley(SD)rats were divided into five groups:Shenmai injection group,citicoline(CDPC) group,0.9% sodium chloride(HIBD-3) group,the sham group,and control group.In the first three groups,the animals were intraperitoneally injected with Shenmai injection,CDPC,HIBD-3 respectively each day for 14 days after the HIBD modeles were completed.Y-maze was used to explore the animals’ behaviors of discrimination learning at 60d and 90d in all five groups.After 24h,the same method was used to explore the animals’ remembrance.Results Compared with HIBD-3 group,the animals’ behaviors of discrimination learning and memory after 24h were significantly different in Shenmai group and CDPC group(P<0.05);There was also statisticaly different between Shenmai group and CDPC group(P<0.05).Conclusion Both the CDPC and Shenmai injection can improve the learning and remembering ability of rat with HIBD,the later is better than the former.

参考文献：

[1] RICE J E, VANNUCCI R C, BRIERLEY J B. The influence of immaturity on hypoxic-ischemic brain damage in the rat. 1981. doi:10.1002/ana.410090206
[2] NAGATA N, SAJI M, ITO T. Repetitive intermittent hypoxia-ischemia and brain damage in neonatal rats, 2000(5)
[3] NORTHINGTON F J, FERRIERO D M, GRAHAM E M. Early Neurodegeneration after hypoxia-ischemia in neonatal rat Is necrosis while delayed neuronal death is apoptosis. 2001(2). doi:10.1006/nbdi.2000.0371
[4] ZHANG Z G, XU X L, SHEN X Y. Efect of fluoride exposure on synaptic structrue of brain areas related to learning memory in mice, 1999(4)
[5] KAKIHANA M, FUKUDA N, SUNO M. Effects of CDP-choline on neurologic deficits and cerebral glucose metabolism in a rat model of cerebral ischemia, 1988
[6] KRUPINSKI J, FERRER I, BARRACHINA M. CDP-choline reduces pro-caspase and cleaved caspase-3 expression,nuclear DNA fragmentation,and specific PARP-cleaved products of caspase activation following middle cerebral artery occlusion in the rat. 2002(6). doi:10.1016/S0028-3908(2)00032-1
[7] KOPF S R, BUCHHOLZER M L, HILGERT M. Glucose plus choline improve passive avoidance behaviour and increase hippocampal acetylcholine release in mice. 2001(2). doi:10.1016/S0306-4522(1)00007-0
[8] 张根明, 孙塑伦, 朱陵群. 参麦注射液对缺氧/缺糖/复氧诱导神经细胞损伤的影响. 中国中医药信息杂志2004(12). doi:10.3969/j.issn.1005-5304.2004.12.010
[9] 童惠贞. 参麦注射液的药理实验研究. 中药材2004(10)
[10] 于凤霞, 李翠萍. 参麦注射液防治心脏缺血再灌注损伤的临床疗效与机理. 医学理论与实践2004(10). doi:10.3969/j.issn.1001-7585.2004.10.015
[11] 姜正林, 吴新民, 陈云. 人参皂甙的抗鼠脑缺氧损伤作用. 中华航海医学杂志2000(1). doi:10.3760/cma.j.issn.1009-6906.2000.01.009
[12] CHOI S R, SAJI H, LIDA Y. Ginseng pretreatment protects against transient global cerebral ischemia in the rat:measurement of local cerebral glucose utilization by[14C] deoxyglucose autoradiography, 1996
[13] 吴新民, 姜正林, 陈益仁. 人参皂甙抗缺血缺氧性脑损伤作用与抑制谷氨酸的释放有关. 南通医学院学报2000(4). doi:10.3969/j.issn.1674-7887.2000.04.009

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录