Objective To evaluate the effect of alanyl-glutamine(Ala-Gln) supplemented of total parenteral nutrition(TPN) on intestinal mucosa and protein metabolism in burned rats.Methods Thirty-three SD rats were divided into three groups equally:chow group,conventional TPN group(convention group) and Ala-Gln supplemented TPN group(Ala-Gln group).The chow group received standard chow; the two TPN groups having been placed a central venous catheter subjected to 30% TBSA Ⅲ° burn and received isocaloric(780 kJ·kg-1·d-1 ) and isonitrogenous(N1.8 g ·kg-1·d-1,48% of nitrogen was supplied with Ala-Gln in Ala-Gln group) TPN for 7 days.Nitrogen balance(NB) and cumulated nitrogen balance(CNB) were monitored.At the end of TPN,serum total protein(TP),albumin(ALB),prealbumin(PAB),transferrin(TRF),Gln in muscle were monitored.Jejunal mucosal morphological changes were observed under light microscope.Results Serum PAB,TRF,Gln level in muscle and CNB in Ala-Gln group were significantly higher than those in convention group(P<0.01).The jejunal villus height,crypt depth,mucosal thickness and villous surface area increased significantly in Ala-Gln group.Conclusion Ala-Gln supplemented TPN in burned rats can promote protein anabolism,improve nitrogen balance,attenuate the decline in Gln in muscle and intestinal mucosal atrophy. |