用碳二亚胺(EDC)法将14位羟基修饰的雷公藤内酯醇(TP)和不同的蛋白栽体(阳离子化牛血清白蛋白、鸡卵清蛋白)偶联合成TP的人工免疫抗原和检测抗原,紫外光谱鉴定偶联效果,计算偶联率.利用免疫抗原免疫小鼠,制备小鼠多克隆抗体,用检测抗原分析血清抗体效价,利用抗原竞争EUSA分析抗体特异性,为进一步研究TP的分子作用机理以及制备TP的单克隆抗体奠定基础.

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