Objective To prepare the rabbit antibody against Trio protein.Methods Trio cDNA was amplified by RT-PCR,and then was subcloned into the fusion expression vectors pET28a(+) and pGEX-4T-1(His)6C.After being expressed in E.coli BL21,the truncated Trio protein was purifed and used to immunize rabbit.Purified antibody were obtained through affinity chromatography column with the expressed GST-Trio protein.The specificity of the purified antibody was characterized by Western blot.Results The trio gene was successfully inserted into pET28a(+) and pGEX-4T-1(His)6C.After induction,the fusion protein was expressed in the form of inclusion body.The purified fusion protein was obtained by affinity chromatography.After immunizing rabbits,the antibody against Trio was obtained,showed that the antibody had a good specificity through Western blot analysis.Conclusion The rabbit antibody against Trio has been successfully prepared,which lays the foundation for further study of it’s function.