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细胞膜钙ATP酶在心肌缺血/再灌注和腺苷诱导预适应中的作用
作者:奚群英 祝宝华 刘晨 张能锋 
单位:东南大学医学院,附属扬州医院,江苏,扬州,225001
关键词:钙ATP酶  再灌注损伤 药物预处理 大鼠 Sprague-Dawley 
分类号:R54-33, R962
出版年·卷·期(页码):2008·27·第四期(238-243)
摘要:

目的:探讨在心肌缺血/再灌注和腺苷诱导预适应过程中细胞膜钙泵(PMCA)的可能作用.方法:将培养的乳鼠心肌细胞分为3组:缺血/再灌注组、腺苷预处理组和对照组.各组乳酸脱氢酶(LDH)漏出量以生化法检测(n=5);半定量RT-PCR检测各组PMCA基因转录水平(n=3);PMCA活性以定磷比色法测定(n=5).结果:(1)缺血/再灌注组LDH漏出量明显增高(P<0.001),腺苷预处理组LDH漏出量显著降低(P<0.001);(2)各组间PMCA mRNA转录水平无显著性差异(P>0.05);(3)各组间PMCA蛋白活性无显著性差异(P>0.05).结论:在乳鼠心肌细胞缺血/再灌注过程中PMCA无代偿功能;PMCA不直接参与腺苷诱导预适应的心肌保护作用.

参考文献:

[1] GUPPY L J, LITTLETON J M. Increased sensitivity to damaging effects of hypoxia and anoxia of isolated hearts from rats after prolonged exposure to ethanol:apparent protection by nitrendipine, 1999(5)
[2] ISSEI K, MASASHI O. Forefront of Na+/Ca2+ exchanger studies:role of Na+/Ca2+ exchanger -lessons from knockout mice. 2004(1). doi:10.1254/jphs.FMJ04002X5
[3] NAWADA R, MURAKAMI T, IWASE T. Inhibition of sarcolemmal Na+,K+-ATPase activity reduces the infart size-limiting effect of preconditioning in rabbit hearts. 1997
[4] YOSHIYAMA M, NAKAMURA Y, OMURA T. Cardioprotective effect of SEA0400,a selective inhibitor of the Na(+)/Ca(2+) exchanger,on myocardial ischemia-reperfusion injury in rats. 2004(2). doi:10.1254/jphs.FPJ03101X
[5] REUTER H, HENDERSON S A, HAN T. Cardiac excitation-contraction coupling in the absence of Na(+)-Ca2+ exchange, 2003(1)
[6] HENDERSON S A, GOLDHABER J I, SO J M. Functional adult myocardium in the absence of Na+-Ca2+ exchange:cardiac-specific knockout of NCX1. 2004(6). doi:10.1161/01.RES.0000142316.08250.68
[7] KAWABATA K I, NETTICADAN T, OSADA M. Mechanisms of ischemic preconditioning effects on Ca2+ paradox-induced changes in heart, 2000
[8] MUBAGWA K, FLAMENG W. Adenosine,adenosine receptors and myocardial protection:an update overview. 2001. doi:10.1016/S0008-6363(1)00358-3
[9] BAXTER G F. Role of adenosine in delayed preconditioning of myocardium. 2002(3). doi:10.1016/S0008-6363(2)00280-8
[10] XIA Y, RAJAPUROHITAM V, COOK M A. Inhibition of phenylephrine induced hypertrophy in rat neonatal cardiomyocytes by the mitochondrial KATP channel opener diazoxide. 2004(5). doi:10.1016/j.yjmcc.2004.07.002
[11] DANILO G, FABRIZIO G, ERNESTO C. Differential membrane targeting of the SERCA and PMCA calcium pumps:experiments with recombinant chimeras, 2002
[12] ERNESTO C. Biogenesis:plasma membrane calcium atpase:15 years of work on the purified enzyme, 1994
[13] CHOI H S, EISNER D A. The role of sarcolemmal Ca2+-ATPase in the regulation of resting calcium concentration in rat ventricular myocytes. 1999(Pt 1). doi:10.1111/j.1469-7793.1999.109ad.x
[14] SAMOUILIDOU E C, KARLI J N, LEVIS G M. The sarcolemmal Ca2+-ATPase of the ischemic-reperfused myocardium:protective effect of hypocalcemia on calmodulin-stimulated activity. 1998(1). doi:10.1016/S0024-3205(97)01035-7
[15] MATSUBARA T, DHALLA N S. Relationship between mechanical dysfunction and depression of sarcolemmal Ca(2+)-pump activity in hearts perfused with oxygen free radicals. 1996(1). doi:10.1007/BF00240048
[16] STREHLER E E, ZACHARIAS D A. Role of alternative splicing in generating isoform diversity among plasma membrane calcium pumps. 2001
[17] MACKIEWICZ U, LEWARTOWSKI B. Temperature dependent contribution of Ca2+ transporters to relaxation in cardiac myocytes:important role of sarcolemmal Ca2+-ATPase, 2006(1)
[18] BERS D M, BASSANI J W M, BASSANI R A. Competition and redistribution among calcium transport systems in rabbit cardiac myocytes. 1993. doi:10.1093/cvr/27.10.1772
[19] BASSANI J W M, BASSANI R A, BERS D M. Relaxation in rabbit and rat cardiac cells:species dependent differences in cellular mechanisms, 1994
[20] SHULL G E. Gene knockout studies of Ca2+-transporting ATPases. 2000(17). doi:10.1046/j.1432-1327.2000.01568.x
[21] BRINI M, BANO D, MANNI S. Effects of PMCA and SERCA pump overexpression on the kinetics of cell Ca2+ signalling. 2000(18). doi:10.1093/emboj/19.18.4926

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