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细胞膜钙ATP酶在心肌缺血/再灌注和腺苷诱导预适应中的作用
作者:奚群英 祝宝华 刘晨 张能锋 
单位:东南大学医学院,附属扬州医院,江苏,扬州,225001
关键词:钙ATP酶  再灌注损伤 药物预处理 大鼠 Sprague-Dawley 
分类号:R54-33, R962
出版年·卷·期(页码):2008·27·第四期(238-243)
摘要:

目的:探讨在心肌缺血/再灌注和腺苷诱导预适应过程中细胞膜钙泵(PMCA)的可能作用.方法:将培养的乳鼠心肌细胞分为3组:缺血/再灌注组、腺苷预处理组和对照组.各组乳酸脱氢酶(LDH)漏出量以生化法检测(n=5);半定量RT-PCR检测各组PMCA基因转录水平(n=3);PMCA活性以定磷比色法测定(n=5).结果:(1)缺血/再灌注组LDH漏出量明显增高(P<0.001),腺苷预处理组LDH漏出量显著降低(P<0.001);(2)各组间PMCA mRNA转录水平无显著性差异(P>0.05);(3)各组间PMCA蛋白活性无显著性差异(P>0.05).结论:在乳鼠心肌细胞缺血/再灌注过程中PMCA无代偿功能;PMCA不直接参与腺苷诱导预适应的心肌保护作用.

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