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缺氧对小鼠背根神经节细胞IK电流的影响
作者:李爱1 曹雪松2 曹雪红1 高琳琳1 胡新武1 张亮品1 陈蕾1 刘烈炬1 刘长金1 
单位:1.华中科技大学同济医学院,生理学系,湖北,武汉,430030; 2.安庆市立医院,安徽,安庆,246000
关键词:背根神经节小细胞 缺氧 电压门控钾通道 全细胞膜片钳 
分类号:R33-33, Q952.5
出版年·卷·期(页码):2008·27·第四期(233-237)
摘要:

目的:探讨氧感受过程参与对钾通道调制的假设.方法:应用新鲜分离的小鼠背根神经节小细胞,采用全细胞膜片钳记录IK电流.结果:当小细胞缺氧时,钾通道在3 min之内即可发生变化,表现为绝大多数(86%, 6/7)细胞的IK减弱,1个细胞(14%,1/7)的IK电流增强;缺氧1 min时IK减弱,3 min时产生最大抑制.当测试电位从+10 mV至+70 mV时,急性缺氧显著性降低IK,IK电流密度降低最大幅度从(304.4±122.9)降为(253.9±106.4)pA·pF-1,但IK稳态激活曲线无明显变化.结论:急性缺氧抑制小鼠背根神经节小细胞IK电流,而IK电流的抑制作用可能是外周神经细胞对缺氧的一种适应和保护机制.

参考文献:

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