兔纤维环细胞和骨髓间充质干细胞共培养的初步研究-东南大学学报医学版

兔纤维环细胞和骨髓间充质干细胞共培养的初步研究

单位：东南大学附属中大医院,骨科,江苏,南京,210009

分类号：R-33, Q813.11

出版年·卷·期（页码）：2008·27·第三期（195-198）

摘要：

目的:初步探讨骨髓间充质干细胞(BMMSCs)与纤维环(AF)细胞的共培养对细胞增殖及主要细胞外基质的影响,进一步确定BMMSCs移植对椎间盘退变的预防和治疗作用.方法:分别培养兔的BMMSCs与AF细胞,将两种细胞按1∶1的比例混匀,利用离心管培养技术培养3周后取材,大体观察并行HE染色;使用荧光染料Hoechst33258检测细胞增殖;通过实时荧光PCR对Ⅱ型胶原、蛋白多糖含量进行测定.结果:在体外培养3周后,细胞团体积达到最大,涂片染色显示共培养组细胞致密,细胞增殖较对照组明显,实时荧光PCR检测结果显示Ⅱ型胶原及蛋白多糖mRNA表达增强.结论:在离心管内三维培养条件下,BMMSCs与AF细胞共培养能够促进增殖,增加Ⅱ型胶原和蛋白多糖的含量.

Objective To explore the effect of the coculture of rabbit bone marrow mesenchymal stem cells(BMMSCs) and anulus fibrosus cells on the proliferation and main extracellular matrix of cells and to evaluate the preventative and therapeutical effect of mesenchymal stem cells on intervertebral disc degeneration.Methods The three groups of cells were cultured in centrifuge tubes after centrifugation.The cellular morphological and functional characteristics were observed using histological,fluorometric assay and real time PCR technique.Results The diameter of cell pellets was reached the maximum within 3 weeks.Cells in cocultured pellets were condensed and proliferated.The higher mRNA expression of typeⅡcollagen and proteoglycans was identified within the cocultured pellets.Conclusion The cocultured system of BMMSCs and anulus fibrosus cells promotes cell proliferation and results in an up-regulation of type Ⅱcollagen and proteoglycans synthesis.

参考文献：

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