环巴胺对胃癌细胞BGC-823增殖凋亡及Shh、Gli-1基因表达的影响-东南大学学报医学版

环巴胺对胃癌细胞BGC-823增殖凋亡及Shh、Gli-1基因表达的影响

单位：东南大学附属中大医院,肿瘤中心,江苏,南京,210009

分类号：Q253, Q255, Q786, R735.2

出版年·卷·期（页码）：2008·27·第三期（188-191）

摘要：

目的:探讨环巴胺对胃癌细胞BGC-823增殖凋亡及Shh、Gli-1基因表达的影响.方法:采用MTT法检测环巴胺对BGC-823细胞的生长抑制效应,倒置显微镜及AO/EB荧光显微镜观察细胞凋亡的形态学改变,流式细胞术检测细胞凋亡率,RT-PCR检测Shh、Gli-1 mRNA的表达情况.结果:环巴胺可明显抑制BGC-823细胞的生长,呈时间剂量依赖性,并出现典型的细胞形态学改变;经32、64、96μmol ·L-1的环巴胺作用24h后的凋亡率依次为12.50%、31.50%、 56.10%,明显高于空白对照组的2.10% (均P<0.01);Shh及Gli-1 mRNA均表达,环巴胺可下调Gli-1 mRNA表达,但对Shh mRNA表达无明显影响.结论:环巴胺可以抑制胃癌细胞BGC-823增殖并诱导细胞凋亡,其机制可能与下调Gli-1基因表达有关.

Objective To investigate effect of cyclopamine on proliferation,apoptosis of gastric carcinoma cell line BGC-823 and expression of Shh and Gli-1 genes.Methods MTT assay was applied to evaluate cell proliferation inhibition rate.Inverted and AO/EB fluorescence microscopes were used to observe the morphologic change.Flow cytometric analysis was used to determine the apoptosis rate.RT-PCR was used to measure the expression of Shh and Gli-1 mRNA.Results Cyclopamine inhibited BGC-823 cell growth significantly,which acted in dose-dependent and time-dependent manners.Different morphologic alterations were found.The cell apoptosis rates after treated with 32,64,96μmol·L-1 cyclopamine for 24h were 12.50%,31.50%,56.10%,respectively,higher than control group(P<0.01).Shh and Gli-1 mRNA all expressed in BGC-823 cells,cyclopamine could decrease the expression of Gli-1 mRNA,instead of not Shh.ConclusionCyclopamine inhibits proliferation and induces apoptosis of BGC-823 cells,through down-regulating the expression of Gli-1 gene.

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