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| 干细胞移植延缓椎间盘退变及磁共振示踪研究 |
| 作者：鲍军平 吴小涛 王运涛 张福勇 朱磊 |
| 单位：东南大学附属中大医院,骨科,江苏,南京,210009 |
| 关键词：椎间盘退变 髓核 骨髓间充质干细胞 干细胞移植 超顺磁性氧化铁 |
| 分类号：R681.55, R-33, Q813, R445.2 |
| 出版年·卷·期（页码）：2008·27·第三期（166-170） |
| 摘要： |
目的:评价超顺磁性氧化铁纳米粒子标记的骨髓间充质干细胞(BMMSCs)在椎间盘退变早期植入椎间盘后细胞外基质的表达情况,并用磁共振进行干细胞示踪.方法:采取体外细胞培养技术,体外纯化扩增兔BMMSCs,并用氧化铁纳米粒子进行标记,于退变手术当时植入手术节段椎间盘内.分别于2、4、6、8周用间苯三酚分光光度法测定蛋白多糖含量的变化,免疫组化法测定Ⅱ型胶原的含量变化,用磁共振扫描对标记干细胞在椎间盘内分布进行示踪.所得数据进行统计学处理.结果:8周内实验组髓核蛋白多糖和Ⅱ型胶原的含量明显较模型组高,差异有统计学意义,但与正常组比较差异无统计学意义.结论:兔BMMSCs移植可以延缓甚至阻止髓核退变. |
Objectve To evaluate the therapeutic efficacy of transplanting bone marrow mesenchymal stem cells(BMMSCs) that increase the amount of proteoglycan and collagen typeⅡcontent in early degenerating intervertebral discs.Methods An in vivo model was established to investigate the feasibility of marrow mesenchymal stem cells that cultured in vitro and marked with SPIO retention,and survived in disc space.In 2,4,6,8 weeks,immunohistochemical staining was used to determine the change of collagen type Ⅱcontent;spectrophotometry to determine the change of amount of proteoglycan with phlorglucinol.Magnetic resonance scanning was also performed to trace the implanted cells in discs.Result BMMSCs could maintain viability and proliferate in the rabbit intervertebral disc.The amount of proteoglycan and collagen type Ⅱcontent of the intervertebral in matrix synthesis in the experiment group was increased in 8 weeks.No changes were found in the model group.Conclusion Transplanted mesenchymal stem cells can delay or even prevent the degeneration of the intervertebral disc.The transplanted cells can migrate in the discs. |
| 参考文献： |
[1] SOBAJIMA S, SHIMER A, KIM J. Feasibility of stem cell therapy for intervertebral disc degeneration. 2004. doi:10.1016/j.spinee.2004.05.239 |
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