p16基因在K562/A02细胞株表达情况的研究-东南大学学报医学版

p16基因在K562/A02细胞株表达情况的研究

作者：寿倍明¹ 陈宝安² 周冬蕊³ 刘德龙⁴ 丁家华² 高冲² 孙耘玉² 王骏² 程坚² 赵刚² 宋慧慧² 鲍文² 陆祖宏³

单位：1.东南大学,临床医学院,江苏,南京,210009； 2.东南大学附属中大医院,血液科,江苏,南京,210009； 3.东南大学,生物科学与医学工程学院,江苏,南京,210096； 4.纽约医学院,医学部,美国,纽约,10595

分类号：Q786, R733.72

出版年·卷·期（页码）：2008·27·第三期（151-153）

摘要：

目的:研究p16基因在K562/A02细胞株的表达情况及其与耐药的关系.方法:用PCR法对慢性髓系白血病细胞株K562及其耐药细胞株K562/A02进行p16基因缺失检测.结果:K562及K562/A02细胞株经PCR扩增后,均未扩出相应条带.结论:K562/A02细胞株与K562细胞株一样,均表现为p16基因缺失.

Objective To discuss the expression of p16 gene in K562/A02 cell line and the relationship between the expression of p16 gene and multidrug resistance.Method Polymerase chain reaction method was used to detect the expression of p16 gene.Result After PCR,both K562 and K562/A02 cell line had not been found to have amplified stripes.Conclusion Both K562 and K562/A02 cell line show deletion of p16 gene.

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