Objective To explore the biological effect of angiopoietin-1(Ang1)and the interaction of Ang1 and VEGF165 on human gastric cancer cell line MGC-803,and study their mechanism in tumorigenesis.Methods The cell line MGC-803 was transfected with Ad-GFP,Ad-Ang1,Ad-VEGF165 and Ad-Ang1+Ad-VEGF165/2.The proliferation and apoptosis of MGC-803 was measured by MTT colorimetry and flow cytometer respectively.Expression of Ki-67 was measured by immunocytochemistry.The expression of bcl-2 mRNA,bax mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results Ang1,VEGF165 and Ang1+VEGF165/2 transfected MGC-803 successfully.Proliferations of MGC803 cells cultured in Ad-Ang1 group,Ad-VEGF165 group and Ad-Ang1+Ad-VEGF165/2 group were higher than Ad-GFP group and controlled group(P<0.05).Proliferation of MGC-803 cells cultured in Ad-Ang1+ Ad-VEGF165/2 group was higher than Ad-Ang1 group and Ad-VEGF165 group(P<0.05).Ang1,VEGF165 and Ang1+VEGF-165/2 could significantly inhibit cell apoptosis comparing with Ad-GFP group and controlled group(P<0.01).Comparing with Ad-GFP group and controlled group,Ki-67 expressing intensity in every tranfected group greatly increased(P<0.01).Expression of bcl-2 mRNA increased in every tranfected group(P<0.01) and expression in Ad-Ang1+ Ad-VEGF165/2 group was strongest.Expression of bax mRNA was reverse.Conclusions Ang1,VEGF165 and Ang1+VEGF165/2 can significantly promote proliferation and inhibit apoptosis of human gastric cancer cell line MGC 803 in vitro.The effect of Ang1+VEGF165/2 on gastric cancer cell line MGC-803 is stronger than Ang1 and VEGF165. |