Objective To investigate the primary culture technique in vitro,which is convenient,economic and pragmatic,for corneal epithelial cells from rabbit′s corneal materials.Methods The primary corneal epithelial cells were obtained by either digestion or tissue culture.The morphological characteristics of culture cells of both methods were observed by inverted phase contrast microscope at various time periods respectively.And the auxodrome(by MTT reduction assay),mitotic index and adherence rate of cells were investigated as well.Results Morphological characteristics of cultured corneal epithelial cells from both methods were resembled in primary and first passage.Comparing to digestion,the cells cultured from tissue culture possessed better proliferative ability in logarithmic phase(P<0.05),and the adherence rate of cells from tissue culture was higher from the 16th hour(P<0.05).Conclusion Tissue culture is a simple,convenient and practicable method for culturing corneal epithelial cells which could be serially subcultivated. |
