紫杉醇埋植剂对小鼠H22肝癌细胞淋巴结转移的治疗作用-东南大学学报医学版

紫杉醇埋植剂对小鼠H22肝癌细胞淋巴结转移的治疗作用

单位：1.东南大学临床医学院,江苏,南京,210009； 2.东南大学附属中大医院,普外科,江苏,南京,210009； 3.东南大学附属中大医院,药剂科,江苏,南京,210009； 4.江苏省药物研究所,江苏,南京,210000

分类号：R-332, R735.7

出版年·卷·期（页码）：2008·27·第一期（36-39）

摘要：

目的:对比研究紫杉醇注射液和紫杉醇埋植剂对小鼠H22肝癌细胞淋巴结转移的治疗作用.方法:将小鼠随机分为无治疗组、空白埋植剂组、紫杉醇注射液组和紫杉醇埋植剂组.治疗12 d,各组一半小鼠取淋巴结称质量并行组织学病理检查,另一半取淋巴结制成无菌匀浆,接种于正常小鼠的腹腔,进行生存分析.结果:无治疗组小鼠淋巴结质量为(96.9±16.4)mg,空白埋植剂组为(95.4±13.6)mg,紫杉醇注射液组为(78.4±14.2)mg,紫杉醇埋植剂组为(32.3±9.4)mg.无治疗组半数生存期为15 d,空白埋植剂组为15.5 d,紫杉醇注射液组为23.5 d,紫杉醇埋植剂组为28 d.结论:紫杉醇埋植剂对淋巴结转移性肿瘤有较好的疗效.

Objectives To investigate the therapeutic effect of paclitaxel and paclitaxel-implants on lymph node metastasis of H22 Hepatoma carcinoma cell.Methods The experimental mice were divided into four groups randomly,group A:no treatment;group B:control implants;group C:paclitaxel;group D:paclitaxel-implants.All mice were sacrificed on day 12 after the treatment,half of the left inguinal lymph nodes were then weighed and fixed in 10% formalin for histologic examination,the rest were minced under aseptic conditions and transferred intraperitoneally to normal recipient mice for the estimation of the mean survival time of tumor recipients.Results The quality of left inguinal lymph nodes was(96.9±16.4) mg in group A,(95.4±13.6) mg in group B,(78.4±14.2) mg in group C and(32.3±9.40) mg in group D;the mean survival time of tumor recipients was 15 d in group A,15.5 d in group B,23.5 d in group C and 28 d in group D.Conclusion Subcutaneous implantation of paclitaxel-implants is effective for the treatment of lymph node metastasis in mice for their high lymphatic uptakes and sustained release.

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