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高脂饲养小鼠糖耐量异常的机理研究
作者:吕红梅  郗有丽  刘桦 
单位:东南大学医学院 药理学教研室, 江苏 南京 210009
关键词:高脂饲养小鼠 骨骼肌 葡萄糖耐受 Akt TBC1D1 
分类号:R-33
出版年·卷·期(页码):2013·32·第六期(746-750)
摘要:

目的:研究高脂饲养对小鼠骨骼肌胰岛素信号通路的影响,并对其葡萄糖耐受机制进行初步探讨。方法:C57/B6J雄性小鼠随机分为正常对照组、高脂饲养组2组,分别进食正常饲料和高脂饲料,6周后检测小鼠空腹血糖、血脂并进行糖耐量试验。应用Western blotting检测骨骼肌中Akt及其底物TBC1D1信号分子的表达变化。结果:高脂饲养组小鼠血脂升高(P<0.05),空腹血糖及腹腔注射葡萄糖后在0.5、1、2 h 3个时间点的血糖也显著上升(P<0.05),骨骼肌中Akt和TBC1D1磷酸化的表达水平均显著下降(P<0.01)。结论:高脂饲养小鼠存在糖耐量异常,骨骼肌中Akt和TBC1D1的磷酸化表达下降可能是高脂诱导小鼠糖耐量异常的机制之一。

Objective: To study the effect of high-fat diet on insulin signaling pathway in skeletal muscle of mice, and examine the mechanism of abnormal glucose tolerance. Methods: Male C57BL/6J mice were randomized into normal diet group and high-fat diet group. Blood lipid, fast blood glucose, and glucose tolerance were measured after 6 weeks. Total and phosphorylated proteins of Akt and TBC1D1 in skeletal muscle were determined via immunoblotting. Results: Fast blood glucose and blood lipid in high-fat diet group were obviously increased(P<0.05). The blood glucose also increased significantly in 0.5, 1, 2 h after intraperitoneal injection of glucose(P<0.05), while phosphorylated proteins of Akt and TBC1D1 in skeletal muscle significantly decreased(P<0.01). Conclusion: Abnormal glucose tolerance exists in high-fat diet mice. The decreased expression of phosphorylated Akt and TBC1D1 in skeletal muscle may be associated with abnormal glucose tolerance induced by high-fat diet.

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