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神经递质多巴胺干预MDSC的功能及影响肿瘤生长的研究
作者:吴瑾  唐宁  龚自珍  周洁菲  蔡威 
单位:上海交通大学医学院附属新华医院 上海市儿科医学研究所, 上海 200092
关键词:多巴胺 髓源性抑制细胞 肿瘤 T细胞增殖 
分类号:R392.12
出版年·卷·期(页码):2013·32·第六期(723-727)
摘要:

目的:研究神经递质多巴胺对肿瘤单核细胞性髓源性抑制细胞(MDSC)抑制功能的干预作用及其对肿瘤生长的影响。方法:体内注射多巴胺,观察对肺癌小鼠模型肿瘤生长的影响;采用流式细胞术检测多巴胺注射对荷瘤鼠骨髓单核细胞性MDSC数量及细胞表型的影响;以T细胞增殖实验检测多巴胺处理对MDSC抑制功能的影响。结果:多巴胺注射可延缓肿瘤生长、明显降低单核细胞性MDSC对T细胞增殖的抑制作用,并促进MDSC的分化成熟。结论:神经递质多巴胺可干预肿瘤单核细胞性MDSC的抑制功能,可能是其抗肿瘤作用的机制之一。

Objective: To investigate the interference effect of neurotransmitter dopamine on the suppressive function of tumor monocytic myeloid-derived suppressor cell (MDSC) and to explore its possible mechanism. Methods: By the use of lung cancer mouse model, we observed the effect of dopamine administration on the tumor growth. Proliferation assay was performed to assess whether dopamine treatment could modulate suppressive function of tumor-derived monocytic MDSC on T cell proliferation. The influence of dopamine on the quantity and phenotype of monocytic MDSC from tumor bone marrow was determined by flow cytometry. Results: Dopamine administration retarded tumor growth and reduced suppressive effect of monocytic MDSC on T cell proliferation, and also promoted its differentiation and maturation. Conclusion: Neurotransmitter dopamine can modulate the suppressive effect of tumor-derived monocytic MDSC, which may be one possible mechanism contributes to its anti-tumor activity.

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