抗Eppin抗体抑制人精子活性运动及相关机制-东南大学学报医学版

抗Eppin抗体抑制人精子活性运动及相关机制

单位：1. 中国药科大学药学院药理教研室, 江苏南京 210009;
2. 南京医科大学生殖医学重点实验室, 公共卫生学院毒理系, 江苏南京 210029

分类号：R-33

出版年·卷·期（页码）：2013·32·第六期（686-691）

摘要：

目的：阐明抗附睾蛋白酶抑制剂（Eppin）抗体抑制人精子动力及分析相关机制。方法：将正常男性精子经过percoll分选后分为7组：空白对照组，抗体对照组与40 μg·ml^-1抗兔多克隆抗体孵育，其余5组分别与不同浓度抗Eppin抗体（浓度分别为2.5、5、10、20和40 μg·ml^-1）共同孵育4 h后进行精子动力测定，线粒体膜电位（Δψ）、ATP浓度及电镜镜检检测。结果：随着抗Eppin抗体浓度的增加，精子活力下降并呈浓度依赖趋势；当抗Eppin抗体浓度超过5 μg·ml^-1，精子Δψ及产生ATP浓度显著下降（P<0.05）；部分精子线粒体正常环形结构发生异常改变，亚线粒体膜变薄甚至消失。结论：抗Eppin抗体与人精子共孵育后精子线粒体功能及超微结构异常，导致精子活性运动显著下降。

Objective: To clarify the inhibition of sperm activated motility by the anti-Eppin antibody and the potential mechanisms. Methods: Semen samples were obtained by masturbation from normal donors. The motile sperm were collected through discontinuous percoll gradient centrifugation and divided into 7 groups. The sperm in one group were incubated with the 40 μg·ml^-1 anti-rabbit antibody and other five groups were incubated with the 2.5, 5, 10, 20 or 40 μg·ml^-1 anti-Eppin antibody. Results: After the sperm treated with the anti-Eppin antibody, the results of computer-assisted semen analysis, mitochondrial membrane potential (Δψ) together with ATP production assays measurement and the images of electron microscopy show the reduction of the activated motility, Δψ and ATP production, and the aberrations of the helical pattern of mitochondria with the partial loss of sub-mitochondrial reticulum. Conclusion: After the human sperm incubated with the anti-Eppin antibody, the aberrations in ultra structure and the functions of the mitochondria of the sperm result in the reduction of the sperm activated motility.

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