Objective: To analyze the evolution of FADD protein in different species and its transcription and expression distribution characteristics in different tissues. Methods: Protein sequences of FADD in eight main species were compared and analyzed by using the software Mega 5.0, and the neighbor-joining phylogenetic tree was drawn according to the protein sequence similarity. FADD+/- mice were sacrificed and their brain, liver, kidney, heart, lung, muscle, spleen, stomach, intestine were obtained and then divided into two parts. Half of the tissues were used to extract RNA for RT-PCR, the rests were used for western blot analyses. The results of FADD expression in FADD+/- mice were compared with the results of UniGene EST Profile at both mRNA and protein levels. Results: On the one hand, we obtained the protein sequence alignment and evolution law of FADD in different species. On the other hand, we found FADD expression was relatively high in lung, heart, stomach, while the expression of FADD in other tissues remains to be verified. Conclusion: The present studies find that FADD is highly conserved in the process of evolution and it is highly expressed in lung, heart and stomach. These results provide a foundation for further investigation of the biological function of FADD in different tissues. |
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